In Vitro Analysis of the Differentiation Capacity of Postmortally Isolated Human Chondrocytes Influenced by Different Growth Factors and Oxygen Levels.
Aggrecans
/ metabolism
Cartilage, Articular
/ cytology
Cell Culture Techniques
Cell Differentiation
/ physiology
Cells, Cultured
Chondrocytes
/ physiology
Chondrogenesis
Glycosaminoglycans
/ metabolism
Humans
Insulin-Like Growth Factor I
/ metabolism
Knee Joint
/ cytology
Mesenchymal Stem Cells
Oxygen
/ metabolism
Transforming Growth Factor beta1
/ metabolism
3D pellet cultures
cartilage
chondrocytes
chondrogenic differentiation
growth factors
oxygen
Journal
Cartilage
ISSN: 1947-6043
Titre abrégé: Cartilage
Pays: United States
ID NLM: 101518378
Informations de publication
Date de publication:
01 2019
01 2019
Historique:
pubmed:
19
7
2017
medline:
14
8
2019
entrez:
19
7
2017
Statut:
ppublish
Résumé
In the present in vitro study, we analyzed the chondrogenic differentiation capacity of human chondrocytes postmortally isolated from unaffected knee cartilage by the addition of transforming growth factor-β1 (TGF-β1) and/or insulin-like growth factor-1 (IGF-1) and different oxygen levels. After 14 and 35 days, DNA concentrations and protein contents of Col1, Col2, aggrecan as well as glycosaminoglycans (GAGs) of chondrocytes cultivated as pellet cultures were analyzed. Additionally, expression rates of mesenchymal stem cell (MSC)-associated differentiation markers were assessed in monolayer cultures. All cultivated chondrocytes were found to be CD29 Our results underline the substantial chondrogenic potential of human chondrocytes postmortally isolated from unaffected articular knee cartilage especially in case of TGF-β1 administration.
Identifiants
pubmed: 28715962
doi: 10.1177/1947603517719318
pmc: PMC6376569
doi:
Substances chimiques
Aggrecans
0
Glycosaminoglycans
0
IGF1 protein, human
0
TGFB1 protein, human
0
Transforming Growth Factor beta1
0
Insulin-Like Growth Factor I
67763-96-6
Oxygen
S88TT14065
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Pagination
111-119Références
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