Characterization and functional analysis of hsp18.3 gene in the red flour beetle, Tribolium castaneum.
Tchsp18.3
Tribolium castaneum
development
reproduction
stress
Journal
Insect science
ISSN: 1744-7917
Titre abrégé: Insect Sci
Pays: Australia
ID NLM: 101266965
Informations de publication
Date de publication:
Apr 2019
Apr 2019
Historique:
received:
02
06
2017
revised:
23
08
2017
accepted:
04
09
2017
pubmed:
6
10
2017
medline:
15
3
2019
entrez:
6
10
2017
Statut:
ppublish
Résumé
Small heat shock proteins (sHSPs) are diverse and mainly function as molecular chaperones to protect organisms and cells from various stresses. In this study, hsp18.3, one Tribolium castaneum species-specific shsp, has been identified. Quantitative real-time polymerase chain reaction illustrated that Tchsp18.3 is expressed in all developmental stages, and is highly expressed at early pupal and late adult stages, while it is highly expressed in ovary and fat body at the adult period. Moreover, it was up-regulated 4532 ± 396-fold in response to enhanced heat stress but not to cold stress; meanwhile the lifespan of adults in ds-Tchsp18.3 group reduced by 15.8% from control group under starvation. Laval RNA interference (RNAi) of Tchsp18.3 caused 86.1% ± 4.5% arrested pupal eclosion and revealed that Tchsp18.3 played an important role in insect development. In addition, parental RNAi of Tchsp18.3 reduced the oviposition amount by 94.7%. These results suggest that Tchsp18.3 is not only essential for the resistance to heat and starvation stress, but also is critical for normal development and reproduction in T. castaneum.
Identifiants
pubmed: 28980406
doi: 10.1111/1744-7917.12543
pmc: PMC7379568
doi:
Substances chimiques
Heat-Shock Proteins, Small
0
Insect Proteins
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
263-273Subventions
Organisme : Natural Science Foundation of Jiangsu Province
ID : BK2011785
Organisme : National Natural Science Foundation of China
ID : 31572326
Organisme : National Natural Science Foundation of China
ID : 31172146
Informations de copyright
© 2017 The Authors. Insect Science published by John Wiley & Sons Australia, Ltd on behalf of Institute of Zoology, Chinese Academy of Sciences.
Références
J Insect Physiol. 2011 Sep;57(9):1190-7
pubmed: 21708161
FEBS J. 2010 Jan;277(1):174-85
pubmed: 19968716
Lancet. 2003 Aug 9;362(9382):469-76
pubmed: 12927437
Gene. 2016 Jul 10;585(2):196-204
pubmed: 26923187
Insect Biochem Mol Biol. 2014 Oct;53:1-12
pubmed: 25008786
Dev Genes Evol. 2008 Apr;218(3-4):119-26
pubmed: 18392874
Insect Sci. 2017 Jun;24(3):358-370
pubmed: 26678377
Dev Comp Immunol. 2008;32(5):585-95
pubmed: 17981328
Cell Stress Chaperones. 2015 Jan;20(1):23-35
pubmed: 24947609
Cell Mol Life Sci. 2005 Nov;62(21):2460-76
pubmed: 16143830
J Insect Physiol. 2012 Jun;58(6):830-6
pubmed: 22430495
Int J Biochem Cell Biol. 2012 Oct;44(10):1613-21
pubmed: 22502646
Nature. 1998 Aug 6;394(6693):595-9
pubmed: 9707123
Cell Stress Chaperones. 2007 Winter;12(4):364-72
pubmed: 18229455
Mol Cell Biol. 1991 Dec;11(12):5937-44
pubmed: 1944271
J Biol Chem. 2004 Oct 15;279(42):43382-5
pubmed: 15331597
Ann N Y Acad Sci. 2000;926:126-31
pubmed: 11193028
J Insect Physiol. 2009 Mar;55(3):279-85
pubmed: 19133268
Chromosoma. 1996 Sep;105(3):142-9
pubmed: 8781182
Cell Cycle. 2006 Nov;5(22):2592-601
pubmed: 17106261
J Insect Physiol. 2017 Jan;96:64-72
pubmed: 27756555
J Mol Evol. 1995 Mar;40(3):238-48
pubmed: 7723051
Nature. 2008 Apr 24;452(7190):949-55
pubmed: 18362917
Mol Cancer Res. 2010 Oct;8(10):1399-412
pubmed: 20858736
J Mol Evol. 2006 Mar;62(3):257-66
pubmed: 16474980
Pathol Biol (Paris). 2000 Apr;48(3):280-8
pubmed: 10858960
Dev Genes Evol. 2004 Nov;214(11):575-8
pubmed: 15365833
J Insect Physiol. 1974 Oct;20(10):2023-33
pubmed: 4419755
Proc Natl Acad Sci U S A. 2002 Oct 15;99(21):13504-9
pubmed: 12368478
Proc Natl Acad Sci U S A. 1989 May;86(10):3748-52
pubmed: 2498875
Environ Entomol. 2016 Feb;45(1):258-67
pubmed: 26363174
Curr Biol. 2002 Feb 5;12(3):R85-6
pubmed: 11839285
Insect Biochem Mol Biol. 2009 Oct;39(10):717-25
pubmed: 19715761
Cell Stress Chaperones. 2015 Sep;20(5):767-80
pubmed: 26045203
J Mol Biol. 2015 Apr 10;427(7):1537-48
pubmed: 25681016
Cell Stress Chaperones. 2014 Sep;19(5):623-33
pubmed: 24379085
Annu Rev Entomol. 2010;55:207-25
pubmed: 19725772
Int J Biochem Cell Biol. 2012 Oct;44(10):1622-31
pubmed: 22521623
Nat Struct Mol Biol. 2005 Oct;12(10):842-6
pubmed: 16205709
PLoS One. 2015 Jul 21;10(7):e0132700
pubmed: 26196395
Dev Cell. 2004 Aug;7(2):167-78
pubmed: 15296714
Nature. 1973 Jul 13;244(5411):103-5
pubmed: 4583478
Proc Natl Acad Sci U S A. 2007 Jul 3;104(27):11130-7
pubmed: 17522254
Methods. 2001 Dec;25(4):402-8
pubmed: 11846609
J Mol Evol. 2004 Dec;59(6):792-805
pubmed: 15599511
Biochim Biophys Acta. 2015 Apr;1854(4):291-319
pubmed: 25556000
BMC Evol Biol. 2009 Aug 28;9:215
pubmed: 19715580
Arch Insect Biochem Physiol. 2006 Jun;62(2):80-90
pubmed: 16703614
Genetica. 2008 Jul;133(3):307-19
pubmed: 17940840