Rigor and reproducibility in rodent behavioral research.

Animals Behavioral Research / methods Mice / psychology Rats / psychology Reproducibility of Results Research Design
Behavior Behavioral assays Best practices Cognitive Experimental design Mice Novel object recognition Rats Replication Reproducibility Rigor Statistical analysis

Journal

Neurobiology of learning and memory
ISSN: 1095-9564
Titre abrégé: Neurobiol Learn Mem
Pays: United States
ID NLM: 9508166

Informations de publication

Date de publication:
11 2019
Historique:
received: 01 11 2017
revised: 22 12 2017
accepted: 03 01 2018
pubmed: 9 1 2018
medline: 31 7 2020
entrez: 9 1 2018
Statut: ppublish

Résumé

Behavioral neuroscience research incorporates the identical high level of meticulous methodologies and exacting attention to detail as all other scientific disciplines. To achieve maximal rigor and reproducibility of findings, well-trained investigators employ a variety of established best practices. Here we explicate some of the requirements for rigorous experimental design and accurate data analysis in conducting mouse and rat behavioral tests. Novel object recognition is used as an example of a cognitive assay which has been conducted successfully with a range of methods, all based on common principles of appropriate procedures, controls, and statistics. Directors of Rodent Core facilities within Intellectual and Developmental Disabilities Research Centers contribute key aspects of their own novel object recognition protocols, offering insights into essential similarities and less-critical differences. Literature cited in this review article will lead the interested reader to source papers that provide step-by-step protocols which illustrate optimized methods for many standard rodent behavioral assays. Adhering to best practices in behavioral neuroscience will enhance the value of animal models for the multiple goals of understanding biological mechanisms, evaluating consequences of genetic mutations, and discovering efficacious therapeutics.

Identifiants

DOI: 10.1016/j.nlm.2018.01.001 PMID: 29307548 PMC: PMC6034984
pubmed: 29307548
pii: S1074-7427(18)30001-7
doi: 10.1016/j.nlm.2018.01.001
pmc: PMC6034984
mid: NIHMS934025
pii:
doi:

Types de publication

Journal Article Research Support, N.I.H., Extramural Review

Langues

eng

Sous-ensembles de citation

IM

Pagination

106780

Subventions

Organisme : NIDA NIH HHS
ID : R01 DA020140
Pays : United States
Organisme : NICHD NIH HHS
ID : U54 HD083091
Pays : United States
Organisme : NIDCD NIH HHS
ID : R01 DC012050
Pays : United States
Organisme : NICHD NIH HHS
ID : U54 HD090256
Pays : United States
Organisme : NICHD NIH HHS
ID : U54 HD090260
Pays : United States
Organisme : NIH HHS
ID : P51 OD010425
Pays : United States
Organisme : NICHD NIH HHS
ID : U54 HD090257
Pays : United States
Organisme : NICHD NIH HHS
ID : U54 HD083092
Pays : United States
Organisme : NINDS NIH HHS
ID : R01 NS085709
Pays : United States
Organisme : NICHD NIH HHS
ID : U54 HD079125
Pays : United States
Organisme : NICHD NIH HHS
ID : U54 HD086984
Pays : United States
Organisme : NICHD NIH HHS
ID : U54 HD090255
Pays : United States

Informations de copyright

Copyright © 2018 Elsevier Inc. All rights reserved.

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Auteurs

Maria Gulinello (M)

IDDRC Behavioral Core Facility, Neuroscience Department, Albert Einstein College of Medicine, Bronx, NY 10461, USA.

Heather A Mitchell (HA)

IDD Models Core, Waisman Center, University of Wisconsin Madison, Madison, WI 53705, USA.

Qiang Chang (Q)

IDD Models Core, Waisman Center, University of Wisconsin Madison, Madison, WI 53705, USA.

W Timothy O'Brien (W)

IDDRC Preclinical Models Core, Children's Hospital of Philadelphia and University of Pennsylvania Perelman School of Medicine, Philadelphia, PA 19104, USA.

Zhaolan Zhou (Z)

IDDRC Preclinical Models Core, Children's Hospital of Philadelphia and University of Pennsylvania Perelman School of Medicine, Philadelphia, PA 19104, USA.

Ted Abel (T)

IDDRC Preclinical Models Core, Children's Hospital of Philadelphia and University of Pennsylvania Perelman School of Medicine, Philadelphia, PA 19104, USA; Current affiliation: Iowa Neuroscience Institute, University of Iowa, Iowa City, IA 52242, USA.

Li Wang (L)

IDDRC Neurobehavioral Core, Center for Neuroscience Research, Children's National Health System, Washington, DC 20010, USA.

Joshua G Corbin (JG)

IDDRC Neurobehavioral Core, Center for Neuroscience Research, Children's National Health System, Washington, DC 20010, USA.

Surabi Veeraragavan (S)

IDDRC Neurobehavioral Core, Baylor College of Medicine, Houston, TX 77030, USA.

Rodney C Samaco (RC)

IDDRC Neurobehavioral Core, Baylor College of Medicine, Houston, TX 77030, USA.

Nick A Andrews (NA)

IDDRC Neurodevelopmental Behavior Core, Boston Children's Hospital, Boston, MA 02115, USA.

Michela Fagiolini (M)

IDDRC Neurodevelopmental Behavior Core, Boston Children's Hospital, Boston, MA 02115, USA.

Toby B Cole (TB)

IDDRC Rodent Behavior Laboratory, Center on Human Development and Disability, University of Washington, Seattle, WA 98195, USA.

Thomas M Burbacher (TM)

IDDRC Rodent Behavior Laboratory, Center on Human Development and Disability, University of Washington, Seattle, WA 98195, USA.

Jacqueline N Crawley (JN)

IDDRC Rodent Behavior Core, MIND Institute, University of California Davis School of Medicine, Sacramento, CA 95817, USA. Electronic address: crawley@ucdavis.edu.

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Classifications MeSH

questionsmedicales.fr › Eucaryotes › Animaux › Rigor and reproducibility in rodent behavioral...
questionsmedicales.fr › Disciplines des sciences naturelles › Science › Recherche › Recherche comportementale › Rigor and reproducibility in rodent behavioral...
questionsmedicales.fr › Eucaryotes › Animaux › Chordés › Vertébrés › Mammifères › Eutheria › Rodentia › Muridae › Murinae › Souris › Rigor and reproducibility in rodent behavioral...
questionsmedicales.fr › Eucaryotes › Animaux › Chordés › Vertébrés › Mammifères › Eutheria › Rodentia › Muridae › Murinae › Rats › Rigor and reproducibility in rodent behavioral...
questionsmedicales.fr › Environnement et santé publique › Santé publique › Méthodes épidémiologiques › Méthodologie en recherche épidémiologique › Reproductibilité des résultats › Rigor and reproducibility in rodent behavioral...
questionsmedicales.fr › Disciplines des sciences naturelles › Science › Recherche › Plan de recherche › Rigor and reproducibility in rodent behavioral...