An approach to testing undiluted e-cigarette aerosol in vitro using 3D reconstituted human airway epithelium.
3D lung tissues
Air-liquid interface
Cytotoxicity
E-cigarette
In vitro
Risk assessment
Undiluted aerosol
Journal
Toxicology in vitro : an international journal published in association with BIBRA
ISSN: 1879-3177
Titre abrégé: Toxicol In Vitro
Pays: England
ID NLM: 8712158
Informations de publication
Date de publication:
Feb 2019
Feb 2019
Historique:
received:
24
11
2017
revised:
11
01
2018
accepted:
15
01
2018
pubmed:
23
1
2018
medline:
15
1
2019
entrez:
23
1
2018
Statut:
ppublish
Résumé
The data presented here show that to provide an estimate of the relative cytotoxicity and therefore potency of e-cigarettes, undiluted aerosol techniques can be used. With the emergence of electronic nicotine delivery systems, fit-for-purpose in vitro screening methods are required. Reconstituted 3D human airway epithelium, was exposed to undiluted aerosols at the air-liquid interface, using a Vitrocell VC 10. TEER, cilia beat frequency and cytotoxic responses were assessed. Using two smoking regimes (ISO and HCI) a 3R4F reference cigarette, produced IC
Identifiants
pubmed: 29355593
pii: S0887-2333(18)30011-0
doi: 10.1016/j.tiv.2018.01.010
pii:
doi:
Substances chimiques
Aerosols
0
Culture Media
0
Flavoring Agents
0
Nicotine
6M3C89ZY6R
Acrolein
7864XYD3JJ
cinnamaldehyde
SR60A3XG0F
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
391-401Informations de copyright
Copyright © 2018 The Authors. Published by Elsevier Ltd.. All rights reserved.