Shedding new light on RhoA signalling as a drug target
FLIM
FRET
Intravital imaging
Rho-GTPases
RhoA
biosensors
breast cancer
extracellular matrix
pancreatic cancer
Journal
Small GTPases
ISSN: 2154-1256
Titre abrégé: Small GTPases
Pays: United States
ID NLM: 101530974
Informations de publication
Date de publication:
07 2020
07 2020
Historique:
pubmed:
20
2
2018
medline:
17
7
2021
entrez:
20
2
2018
Statut:
ppublish
Résumé
The small GTPase RhoA is a master regulator of signalling in cell-extracellular matrix interactions. RhoA signalling is critical to many cellular processes including migration, mechanotransduction, and is often disrupted in carcinogenesis. Investigating RhoA activity in a native tissue environment is challenging using conventional biochemical methods; we therefore developed a RhoA-FRET biosensor mouse, employing the adaptable nature of intravital imaging to a variety of settings. Mechanotransduction was explored in the context of osteocyte processes embedded in the calvaria responding in a directional manner to compression stress. Further, the migration of neutrophils was examined during
Identifiants
pubmed: 29457531
doi: 10.1080/21541248.2018.1438024
pmc: PMC7549666
doi:
Substances chimiques
Pharmaceutical Preparations
0
RhoA protein, mouse
EC 3.6.5.2
rhoA GTP-Binding Protein
EC 3.6.5.2
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
240-247Subventions
Organisme : Cancer Research UK
ID : 15565
Pays : United Kingdom
Références
Sci Rep. 2015 Aug 20;5:13283
pubmed: 26290434
Nat Commun. 2017 Feb 10;8:14396
pubmed: 28186127
PLoS Comput Biol. 2016 May 03;12(5):e1004909
pubmed: 27138333
Nat Rev Cancer. 2002 Dec;2(12):897-909
pubmed: 12459728
Int J Cancer. 2017 May 1;140(9):2101-2111
pubmed: 28109008
Arch Biochem Biophys. 2008 May 15;473(2):106-11
pubmed: 18424256
Nat Rev Mol Cell Biol. 2016 Aug;17(8):496-510
pubmed: 27301673
Sci Transl Med. 2017 Apr 5;9(384):
pubmed: 28381539
Curr Biol. 2001 Nov 27;11(23):1836-46
pubmed: 11728306
PLoS One. 2017 Aug 25;12(8):e0183871
pubmed: 28841710
Elife. 2015 Feb 10;4:e05178
pubmed: 25668746
Cell Rep. 2017 Nov 28;21(9):2458-2470
pubmed: 29186684
J Cell Sci. 2012 Feb 15;125(Pt 4):858-68
pubmed: 22399802
Cancer Res. 2016 Sep 15;76(18):5266-76
pubmed: 27488524
Cancer Res. 2013 Aug 1;73(15):4674-86
pubmed: 23749641
Methods. 2017 Sep 1;128:78-94
pubmed: 28435000
Cancer Cell. 2005 May;7(5):469-83
pubmed: 15894267
Onco Targets Ther. 2013 Dec 18;7:33-43
pubmed: 24379681
J Cell Sci. 2007 Jul 1;120(Pt 13):2272-83
pubmed: 17591691
J Cell Sci. 2018 Mar 6;131(5):
pubmed: 29511095
Gastroenterology. 2018 Mar;154(4):820-838
pubmed: 29287624
Proc Natl Acad Sci U S A. 2013 Dec 24;110(52):21012-7
pubmed: 24324138
Gastroenterology. 2017 Oct;153(4):1054-1067
pubmed: 28642198
EMBO Mol Med. 2017 Feb;9(2):198-218
pubmed: 28031255
Cell. 2011 Nov 23;147(5):992-1009
pubmed: 22118458
Nat Med. 2016 Aug;22(8):851-60
pubmed: 27376576
Nat Rev Cancer. 2014 May;14(5):314-28
pubmed: 24739578
Small GTPases. 2013 Jul-Sep;4(3):141-7
pubmed: 24025634
EMBO Rep. 2012 Oct;13(10):900-8
pubmed: 22964758
Nat Rev Mol Cell Biol. 2014 Dec;15(12):786-801
pubmed: 25415508
Proc Natl Acad Sci U S A. 2010 Jan 5;107(1):246-51
pubmed: 20018721
Nat Cell Biol. 2010 Jan;12(1):47-53; sup pp 1-11
pubmed: 20010816
Sci Rep. 2015 Oct 05;5:14693
pubmed: 26435194
Small GTPases. 2014;5:e27958
pubmed: 24607953
Cancer Res. 2009 Nov 15;69(22):8742-51
pubmed: 19887617
Nat Rev Cancer. 2002 Feb;2(2):133-42
pubmed: 12635176
J Cell Biol. 2003 Jul 21;162(2):223-32
pubmed: 12860967
Nat Commun. 2015 May 14;6:7139
pubmed: 25972253
Nature. 2006 Apr 20;440(7087):1069-72
pubmed: 16547516
Biochim Biophys Acta. 2009 Dec;1796(2):91-8
pubmed: 19327386
Cell Rep. 2014 Mar 27;6(6):1153-1164
pubmed: 24630994
Oncogene. 2015 Feb 19;34(8):1051-7
pubmed: 24632612
Nat Protoc. 2013 Mar;8(3):583-94
pubmed: 23429719
Gastroenterology. 2016 Jul;151(1):180-193.e12
pubmed: 27003603
J Clin Oncol. 2018 Feb 1;36(4):359-366
pubmed: 29232172
Small GTPases. 2020 Sep;11(5):354-370
pubmed: 29455593
Cell. 2009 Dec 24;139(7):1327-41
pubmed: 20064378
Nat Commun. 2018 Jan 17;9(1):254
pubmed: 29343721
Nature. 2012 Nov 15;491(7424):399-405
pubmed: 23103869
Cancer Res. 2011 Feb 1;71(3):747-57
pubmed: 21266354
Cancer Res. 2013 Apr 1;73(7):2357-67
pubmed: 23370328
Cell Rep. 2017 Mar 7;18(10):2464-2479
pubmed: 28273460
Gut. 2018 Dec;67(12):2142-2155
pubmed: 29080858
Sci Signal. 2013 Jul 23;6(285):rs12
pubmed: 23882122
Mol Cell. 2013 Jun 27;50(6):805-17
pubmed: 23747015
Curr Opin Cell Biol. 2015 Oct;36:103-12
pubmed: 26363959
Cell Rep. 2017 Oct 3;21(1):274-288
pubmed: 28978480
Cell Syst. 2016 Jan 27;2(1):38-48
pubmed: 27136688
Cancer Cell. 2014 Feb 10;25(2):181-95
pubmed: 24525234
Small GTPases. 2011 Jul;2(4):239-244
pubmed: 22145098
Nature. 2009 Sep 3;461(7260):99-103
pubmed: 19693013
Proc Natl Acad Sci U S A. 2010 Aug 3;107(31):13648-53
pubmed: 20643964
Sci Transl Med. 2012 Oct 31;4(158):158ra145
pubmed: 23115354