Ex Vivo Imaging and Genetic Manipulation of Mouse Hair Follicle Bulge Stem Cells.
Adenovirus
Bulge stem cells
Clonal assay
Hair follicle
Immunofluorescence
Live cell imaging
Patch assay
Journal
Methods in molecular biology (Clifton, N.J.)
ISSN: 1940-6029
Titre abrégé: Methods Mol Biol
Pays: United States
ID NLM: 9214969
Informations de publication
Date de publication:
2019
2019
Historique:
pubmed:
27
2
2018
medline:
4
6
2019
entrez:
26
2
2018
Statut:
ppublish
Résumé
Stem cells that reside in the bulge of adult mouse hair follicles are a leading model of tissue stem cell research. Ex vivo culturing, molecular and cell biological characterizations, as well as genetic manipulation of fluorescence-activated cell sorting-isolated bulge stem cells offer a useful experimental pipeline to complement in vivo studies. Here we describe detailed methods for culturing, immunostaining, live cell imaging, and adenoviral infection of bulge stem cells for downstream applications such as in vitro clonal and in vivo patch assays.
Identifiants
pubmed: 29478134
doi: 10.1007/7651_2018_136
doi:
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
15-29Subventions
Organisme : NIAMS NIH HHS
ID : R01 AR068074
Pays : United States