In vitro assessment of electrospun polyamide-6 scaffolds for esophageal tissue engineering.
biocompatibility
electrospinning
human mesenchymal stromal cells
polyamide-6
synthetic scaffolds
Journal
Journal of biomedical materials research. Part B, Applied biomaterials
ISSN: 1552-4981
Titre abrégé: J Biomed Mater Res B Appl Biomater
Pays: United States
ID NLM: 101234238
Informations de publication
Date de publication:
02 2019
02 2019
Historique:
received:
10
08
2017
revised:
08
02
2018
accepted:
26
02
2018
pubmed:
1
4
2018
medline:
17
6
2020
entrez:
1
4
2018
Statut:
ppublish
Résumé
Artificial tissue-engineered grafts offer a potential alternative to autologous tissue grafts for patients, which can be traumatic. After decellularizing Papio hamadryas esophagus and studying the morphology and physical properties of the extracellular matrix (ECM), we generated electrospun polyamide-6 based scaffolds to mimic it. The scaffolds supported a greater mechanical load than the native ECM and demonstrated similar 3D microstructure, with randomly aligned fibers, 90% porosity, 29 μm maximal pore size, and average fiber diameter of 2.87 ± 0.95 µm. Biocompatibility studies showed that human adipose- and bone marrow-derived mesenchymal stromal cells (AD-MSC and BMD-MSC) adhered to the scaffold surface and showed some proliferation: scaffold cell coverage was 25% after 72 h of incubation when seeded with 1000 cells/mm
Substances chimiques
Polymers
0
nylon 6
25038-54-4
Caprolactam
6879X594Z8
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
253-268Informations de copyright
© 2018 Wiley Periodicals, Inc.