The applicability of furfuryl-gelatin as a novel bioink for tissue engineering applications.
bilayer sheets
biocompatibility
furfuryl-gelatin
hyaluronic acid
visible-light crosslinkable bioink
Journal
Journal of biomedical materials research. Part B, Applied biomaterials
ISSN: 1552-4981
Titre abrégé: J Biomed Mater Res B Appl Biomater
Pays: United States
ID NLM: 101234238
Informations de publication
Date de publication:
02 2019
02 2019
Historique:
received:
31
10
2017
revised:
22
02
2018
accepted:
14
03
2018
pubmed:
16
4
2018
medline:
17
6
2020
entrez:
16
4
2018
Statut:
ppublish
Résumé
Three-dimensional bioprinting is an innovative technique in tissue engineering, to create layer-by-layer structures, required for mimicking body tissues. However, synthetic bioinks do not generally possess high printability and biocompatibility at the same time. So, there is an urgent need for naturally derived bioinks that can exhibit such optimized properties. We used furfuryl-gelatin as a novel, visible-light crosslinkable bioink for fabricating cell-laden structures with high viability. Hyaluronic acid was added as a viscosity enhancer and either Rose Bengal or Riboflavin was used as a visible-light crosslinker. Crosslinking was done by exposing the printed structure for 2.5 min to visible light and confirmed using Fourier transform infrared spectroscopy and rheometry. Scanning electron microscopy revealed a highly porous networked structure. Three different cell types were successfully bioprinted within these constructs. Mouse mesenchymal stem cells printed within monolayer and bilayer sheets showed viability, network formation and proliferation (∼5.33 times) within 72 h of culture. C2C12 and STO cells were used to print a double layered structure, which showed evidence of the viability of both cells and heterocellular clusters within the construct. This furfuryl-gelatin based bioink can be used for tissue engineering of complex tissues and help in understanding how cellular crosstalk happens in vivo during normal or diseased pathology. © 2018 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater, 107B: 314-323, 2019.
Identifiants
pubmed: 29656592
doi: 10.1002/jbm.b.34123
pmc: PMC6188846
mid: NIHMS953556
doi:
Substances chimiques
Gelatin
9000-70-8
Hyaluronic Acid
9004-61-9
Types de publication
Journal Article
Research Support, N.I.H., Extramural
Research Support, U.S. Gov't, Non-P.H.S.
Langues
eng
Sous-ensembles de citation
IM
Pagination
314-323Subventions
Organisme : NIMHD NIH HHS
ID : G12 MD007592
Pays : United States
Organisme : NHLBI NIH HHS
ID : SC2 HL134642
Pays : United States
Organisme : NIGMS NIH HHS
ID : UL1 GM118970
Pays : United States
Informations de copyright
© 2018 Wiley Periodicals, Inc.
Références
J Cataract Refract Surg. 2007 Mar;33(3):516-21
pubmed: 17321404
Dermatol Ther. 2006 May-Jun;19(3):141-50
pubmed: 16784513
Biofabrication. 2015 Aug 11;7(3):035006
pubmed: 26260872
Tissue Eng. 1999 Dec;5(6):545-54
pubmed: 10611546
Proc Natl Acad Sci U S A. 2015 Feb 17;112(7):1953-8
pubmed: 25646417
Macromol Biosci. 2013 May;13(5):551-61
pubmed: 23420700
Sci Rep. 2016 Aug 31;6:32456
pubmed: 27578567
PLoS One. 2013;8(3):e59012
pubmed: 23527073
Cell. 2009 Nov 25;139(5):891-906
pubmed: 19931152
Cell Biol Int. 2014 Jan;38(1):41-9
pubmed: 24030862
Biomaterials. 2005 Oct;26(29):5864-71
pubmed: 15949552
J Mater Sci Mater Med. 2010 Jun;21(6):1899-911
pubmed: 20238149
Biomaterials. 2003 Nov;24(24):4337-51
pubmed: 12922147
Nanomedicine. 2014 Apr;10(3):491-501
pubmed: 24200522
J Biomed Mater Res A. 2013 Dec;101(12):3457-66
pubmed: 23595953
Bone Miner. 1993 Aug;22(2):147-59
pubmed: 8251766
Mater Sci Eng C Mater Biol Appl. 2017 Apr 1;73:788-797
pubmed: 28183673
Nat Rev Mol Cell Biol. 2006 Mar;7(3):211-24
pubmed: 16496023
Circulation. 2011 May 17;123(19):2083-93
pubmed: 21537003
Science. 1997 Feb 14;275(5302):964-7
pubmed: 9020076
Adv Drug Deliv Rev. 2007 May 30;59(4-5):207-33
pubmed: 17482309
Leuk Res. 1992 Dec;16(12):1165-73
pubmed: 1361210
Biomacromolecules. 2000 Spring;1(1):31-8
pubmed: 11709840
Biomacromolecules. 2011 Apr 11;12(4):851-8
pubmed: 21366287
Physiol Genomics. 2003 Nov 11;15(3):165-76
pubmed: 14612588
Nat Rev Cancer. 2004 Jul;4(7):528-39
pubmed: 15229478
Nat Biotechnol. 2014 Aug;32(8):773-85
pubmed: 25093879
Dev Reprod. 2015 Sep;19(3):119-26
pubmed: 27004268
Adv Healthc Mater. 2016 Jan 7;5(1):108-18
pubmed: 25880725
In Vitro Cell Dev Biol Anim. 2014 Aug;50(7):656-63
pubmed: 24737277
Biomaterials. 2009 Jul;30(20):3371-7
pubmed: 19345991
Biomaterials. 2009 May;30(14):2724-34
pubmed: 19201462
Acta Biomater. 2017 Oct 1;61:41-53
pubmed: 28782725
Sci Rep. 2014 Mar 25;4:4457
pubmed: 24662725
Circ Res. 2014 Jan 17;114(2):354-67
pubmed: 24436431
Biotechnol Bioeng. 2003 May 20;82(4):403-14
pubmed: 12632397
Carbohydr Polym. 2015 Sep 5;128:220-7
pubmed: 26005158
Acta Biomater. 2010 Oct;6(10):4005-10
pubmed: 20580950
J Mater Chem B. 2015 Aug 14;3(30):6368-6376
pubmed: 32262755
Biomater Sci. 2013 Feb 3;1(2):224-230
pubmed: 32481802
Biofabrication. 2016 Oct 07;8(4):045002
pubmed: 27716628
Biofabrication. 2010 Jun;2(2):022001
pubmed: 20811127
Cell Biochem Biophys. 2016 Dec;74(4):527-535
pubmed: 27722948
Tissue Eng. 2007 Oct;13(10):2369-85
pubmed: 17658993
Biomaterials. 1999 Jul;20(14):1339-44
pubmed: 10403052
J Mol Cell Cardiol. 2014 May;70:37-46
pubmed: 24412581
Circ Res. 2010 Jul 9;107(1):35-44
pubmed: 20448218
Biomaterials. 2006 May;27(15):2994-3004
pubmed: 16457881
Biochem Soc Trans. 2015 Jun;43(3):513-8
pubmed: 26009200
PeerJ. 2016 Sep 27;4:e2497
pubmed: 27703850
Ann Biomed Eng. 2016 Jun;44(6):2090-102
pubmed: 27184494
Chem Rev. 2001 Jul;101(7):1869-79
pubmed: 11710233
Nat Commun. 2014 Jun 02;5:3935
pubmed: 24887553
J Bone Miner Res. 2014 Jul;29(7):1575-85
pubmed: 24347469
Nano Lett. 2012 Apr 11;12(4):1831-8
pubmed: 22432413
Biomaterials. 2003 Sep;24(21):3825-34
pubmed: 12818555