Petaloid recombinant peptide enhances in vitro cartilage formation by synovial mesenchymal stem cells.
chondrogenesis
petaloid recombinant peptide
regenerative medicine
scaffold
synovial mesenchymal stem cell (MSC)
Journal
Journal of orthopaedic research : official publication of the Orthopaedic Research Society
ISSN: 1554-527X
Titre abrégé: J Orthop Res
Pays: United States
ID NLM: 8404726
Informations de publication
Date de publication:
06 2019
06 2019
Historique:
received:
02
10
2017
accepted:
27
04
2018
pubmed:
8
5
2018
medline:
21
12
2019
entrez:
9
5
2018
Statut:
ppublish
Résumé
In vitro chondrogenesis of mesenchymal stem cells (MSCs) mimics in vivo chondrogenesis of MSCs. However, the size of the cartilage pellets that can be attained in vitro is limited by current methods; therefore, some modifications are required to obtain larger pellets. Petaloid pieces of recombinant peptide (petaloid RCP) have the advantage of creating spaces between cells in culture. The RCP used here is based on the alpha-1 sequence of human collagen type I and contains 12 Arg-Gly-Asp motifs. We examined the effect and mechanisms of adding petaloid RCP on the in vitro chondrogenesis of human synovial MSCs by culturing 125k cells with or without 0.125 mg petaloid RCP in chondrogenic medium for 21 days. The cartilage pellets were sequentially analyzed by weight, sulfated glycosaminoglycan content, DNA retention, and histology. Petaloid RCP significantly increased the weight of the cartilage pellets: The petaloid RCP group weighed 7.7 ± 1.2 mg (n = 108), whereas the control group weighed 5.3 ± 1.6 mg. Sulfated glycosaminoglycan and DNA contents were significantly higher in the petaloid RCP group than in the control group. Light and transmission electron microscopy images showed that the petaloid RCP formed the framework of the pellet at day 1, the framework was broken by production of cartilage matrix by the synovial MSCs at day 7, and the cartilage pellet grew larger, with diffuse petaloid RCP remaining, at day 21. Therefore, petaloid RCP formed a framework for the pellet, maintained a higher cell number, and promoted in vitro cartilage formation of synovial MSCs. © 2018 The Authors. Journal of Orthopaedic Research® Published by Wiley Periodicals, Inc. J Orthop Res 37:1350-1357, 2019.
Identifiants
pubmed: 29737046
doi: 10.1002/jor.24042
pmc: PMC6585959
doi:
Substances chimiques
Glycosaminoglycans
0
Peptides
0
Recombinant Proteins
0
A73025
268AW7000T
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
1350-1357Informations de copyright
© 2018 The Authors. Journal of Orthopaedic Research® Published by Wiley Periodicals, Inc.
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