Prostate tumor cell exosomes containing hyaluronidase Hyal1 stimulate prostate stromal cell motility by engagement of FAK-mediated integrin signaling.
Autophagosomes
/ metabolism
Cell Adhesion
Cell Communication
Cell Culture Techniques
Cell Line, Tumor
Cell Movement
Enzyme Activation
Exosomes
/ metabolism
Focal Adhesion Kinase 1
/ metabolism
Gene Expression Regulation, Neoplastic
Humans
Hyaluronoglucosaminidase
/ metabolism
Integrins
/ metabolism
Male
Microtubule-Associated Proteins
/ metabolism
Prostatic Neoplasms
/ metabolism
Signal Transduction
Stromal Cells
/ cytology
Up-Regulation
Cell motility
Exosomes
Hyaluronan
Hyaluronidase
Prostate cancer
Stromal-epithelial crosstalk
Journal
Matrix biology : journal of the International Society for Matrix Biology
ISSN: 1569-1802
Titre abrégé: Matrix Biol
Pays: Netherlands
ID NLM: 9432592
Informations de publication
Date de publication:
05 2019
05 2019
Historique:
received:
14
12
2017
revised:
28
03
2018
accepted:
08
05
2018
pubmed:
14
5
2018
medline:
31
8
2019
entrez:
14
5
2018
Statut:
ppublish
Résumé
The hyaluronidase Hyal1 is clinically and functionally implicated in prostate cancer progression and metastasis. Elevated Hyal1 accelerates vesicular trafficking in prostate tumor cells, thereby enhancing their metastatic potential in an autocrine manner through increased motility and proliferation. In this report, we found Hyal1 protein is a component of exosomes produced by prostate tumor cell lines overexpressing Hyal1. We investigated the role of exosomally shed Hyal1 in modulating tumor cell autonomous functions and in modifying the behavior of prostate stromal cells. Catalytic activity of Hyal1 was necessary for enrichment of Hyal1 in the exosome fraction, which was associated with increased presence of LC3BII, an autophagic marker, in the exosomes. Hyal1-positive exosome contents were internalized from the culture medium by WPMY-1 prostate stromal fibroblasts. Treatment of prostate stromal cells with tumor exosomes did not affect proliferation, but robustly stimulated their migration in a manner dependent on Hyal1 catalytic activity. Increased motility of exosome-treated stromal cells was accompanied by enhanced adhesion to a type IV collagen matrix, as well as increased FAK phosphorylation and integrin engagement through dynamic membrane residence of β1 integrins. The presence of Hyal1 in tumor-derived exosomes and its ability to impact the behavior of stromal cells suggests cell-cell communication via exosomes is a novel mechanism by which elevated Hyal1 promotes prostate cancer progression.
Identifiants
pubmed: 29753676
pii: S0945-053X(17)30464-X
doi: 10.1016/j.matbio.2018.05.002
pmc: PMC6230312
mid: NIHMS969071
pii:
doi:
Substances chimiques
Integrins
0
MAP1LC3B protein, human
0
Microtubule-Associated Proteins
0
Focal Adhesion Kinase 1
EC 2.7.10.2
PTK2 protein, human
EC 2.7.10.2
HYAL1 protein, human
EC 3.2.1.35
Hyaluronoglucosaminidase
EC 3.2.1.35
Types de publication
Journal Article
Research Support, N.I.H., Extramural
Langues
eng
Sous-ensembles de citation
IM
Pagination
165-179Subventions
Organisme : NIGMS NIH HHS
ID : P20 GM104320
Pays : United States
Organisme : NCI NIH HHS
ID : P30 CA086862
Pays : United States
Organisme : NCI NIH HHS
ID : R01 CA165574
Pays : United States
Informations de copyright
Copyright © 2018 International Society of Matrix Biology. Published by Elsevier B.V. All rights reserved.
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