Differences in gut microbiota associated with age, sex, and stool consistency in healthy Japanese subjects.
16S rRNA
Bristol stool scale
Gut microbiota
Journal
Journal of gastroenterology
ISSN: 1435-5922
Titre abrégé: J Gastroenterol
Pays: Japan
ID NLM: 9430794
Informations de publication
Date de publication:
Jan 2019
Jan 2019
Historique:
received:
07
05
2018
accepted:
11
06
2018
pubmed:
22
6
2018
medline:
18
12
2019
entrez:
22
6
2018
Statut:
ppublish
Résumé
Human gut microbiota is involved in host health and disease development. Investigations of age-related and sex-related alterations in gut microbiota are limited, and the association between stool consistency and gut microbiota has not been fully investigated. We investigated gut microbiota differences related to age, sex, and stool consistency in healthy Japanese subjects. Two-hundred and seventy-seven healthy Japanese subjects aged 20-89 years were enrolled. Fecal samples were obtained to analyze the gut microbiome. We evaluated the association between stool consistency [Bristol stool scale (BSS)] and gut microbiota. Although there were significant differences in the microbial structure between males and females, the α-diversity of gut microbiota showed no difference between males and females or among age groups. There were significant increases in genera Prevotella, Megamonas, Fusobacterium, and Megasphaera and Bifidobacterium, Ruminococcus, and Akkermansia in males and females, respectively. The ratio of hard stools (BSS types 1 and 2) was higher in females; the ratio of loose stools (BSS type 6) was higher in males. No younger male had BSS type 1 or type 2. Fusobacterium in males was significantly higher in the loose consistency group, and Oscillospira was significantly higher in the hard consistency group in males; Campylobacter, SMB53, and Turicibacter were significantly higher in the hard consistency group in females. Several changes in gut microbiota were associated with age and sex. Stool consistency and gut microbiota associations emphasized the importance of stool consistency assessments to understand intestinal function.
Sections du résumé
BACKGROUND
BACKGROUND
Human gut microbiota is involved in host health and disease development. Investigations of age-related and sex-related alterations in gut microbiota are limited, and the association between stool consistency and gut microbiota has not been fully investigated. We investigated gut microbiota differences related to age, sex, and stool consistency in healthy Japanese subjects.
METHODS
METHODS
Two-hundred and seventy-seven healthy Japanese subjects aged 20-89 years were enrolled. Fecal samples were obtained to analyze the gut microbiome. We evaluated the association between stool consistency [Bristol stool scale (BSS)] and gut microbiota.
RESULTS
RESULTS
Although there were significant differences in the microbial structure between males and females, the α-diversity of gut microbiota showed no difference between males and females or among age groups. There were significant increases in genera Prevotella, Megamonas, Fusobacterium, and Megasphaera and Bifidobacterium, Ruminococcus, and Akkermansia in males and females, respectively. The ratio of hard stools (BSS types 1 and 2) was higher in females; the ratio of loose stools (BSS type 6) was higher in males. No younger male had BSS type 1 or type 2. Fusobacterium in males was significantly higher in the loose consistency group, and Oscillospira was significantly higher in the hard consistency group in males; Campylobacter, SMB53, and Turicibacter were significantly higher in the hard consistency group in females.
CONCLUSIONS
CONCLUSIONS
Several changes in gut microbiota were associated with age and sex. Stool consistency and gut microbiota associations emphasized the importance of stool consistency assessments to understand intestinal function.
Identifiants
pubmed: 29926167
doi: 10.1007/s00535-018-1488-5
pii: 10.1007/s00535-018-1488-5
doi:
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
53-63Subventions
Organisme : Japan Society for the Promotion of Science
ID : 16K09322
Organisme : Japan Society for the Promotion of Science
ID : 16H05289
Organisme : Ministry of Agriculture, Forestry and Fisheries
ID : 28020006
Commentaires et corrections
Type : ErratumIn
Références
Lynch SV, Pedersen O. The human intestinal microbiome in health and disease. N Engl J Med. 2016;375:2369–79.
doi: 10.1056/NEJMra1600266
pubmed: 27974040
Clemente JC, Ursell LK, Parfrey LW, et al. The impact of the gut microbiota on human health: an integrative view. Cell. 2012;148:1258–70.
doi: 10.1016/j.cell.2012.01.035
pubmed: 22424233
pmcid: 5050011
Kedia S, Rampal R, Paul J, et al. Gut microbiome diversity in acute infective and chronic inflammatory gastrointestinal diseases in North India. J Gastroenterol. 2016;51:660–71.
doi: 10.1007/s00535-016-1193-1
pubmed: 26994772
Ley RE, Turnbaugh PJ, Klein S, et al. Microbial ecology: human gut microbes associated with obesity. Nature. 2006;444:1022–3.
doi: 10.1038/4441022a
pubmed: 17183309
Vandeputte D, Falony G, Vieira-Silva S, et al. Stool consistency is strongly associated with gut microbiota richness and composition, enterotypes and bacterial growth rates. Gut. 2016;65:57–62.
doi: 10.1136/gutjnl-2015-309618
pubmed: 26069274
Tigchelaar EF, Bonder MJ, Jankipersadsing SA, et al. Gut microbiota composition associated with stool consistency. Gut. 2016;65:540–2.
doi: 10.1136/gutjnl-2015-310328
pubmed: 26276682
Hadizadeh F, Walter S, Belheouane M, et al. Stool frequency is associated with gut microbiota composition. Gut. 2017;66:559–60.
doi: 10.1136/gutjnl-2016-311935
pubmed: 27196592
Longstreth GF, Thompson WG, Chey WD, et al. Functional bowel disorders. Gastroenterology. 2006;130:1480–91.
doi: 10.1053/j.gastro.2005.11.061
pubmed: 16678561
Degen LP, Phillips SF. How well does stool form reflect colonic transit? Gut. 1996;39:109–13.
doi: 10.1136/gut.39.1.109
pubmed: 8881820
pmcid: 1383242
Consortium THMP. Structure, function and diversity of the healthy human microbiome. Nature. 2012;486:207–14.
doi: 10.1038/nature11234
Arumugam M, Raes J, Pelletier E, et al. Enterotypes of the human gut microbiome. Nature. 2011;473:174–80.
doi: 10.1038/nature09944
pubmed: 21508958
pmcid: 3728647
Nishijima S, Suda W, Oshima K, Kim SW, Hirose Y, Morita H, et al. The gut microbiome of healthy Japanese and its microbial and functional uniqueness. DNA Res. 2016;23:125–33.
doi: 10.1093/dnares/dsw002
pubmed: 26951067
pmcid: 4833420
Nakayama J, Watanabe K, Jiang J, et al. Diversity in gut bacterial community of school-age children in Asia. Sci Rep. 2015;5:8397.
doi: 10.1038/srep08397
pubmed: 25703686
pmcid: 4336934
Heaton KW, Radvan J, Cripps H, et al. Defecation frequency and timing, and stool form in the general population: a prospective study. Gut. 1992;33:818–24.
doi: 10.1136/gut.33.6.818
pubmed: 1624166
pmcid: 1379343
Inoue R, Ohue-Kitano R, Tsukahara T, et al. Prediction of functional profiles of gut microbiota from 16S rRNA metagenomic data provides a more robust evaluation of gut dysbiosis occurring in Japanese type 2 diabetic patients. J Clin Biochem Nutr. 2017;61:217–21.
doi: 10.3164/jcbn.17-44
pubmed: 29203964
pmcid: 5703784
Nishino K, Nishida A, Inoue R, et al. Analysis of endoscopic brush samples identified mucosa-associated dysbiosis in inflammatory bowel disease. J Gastroenterol. 2018;53:95–106.
doi: 10.1007/s00535-017-1384-4
pubmed: 28852861
Takagi T, Naito Y, Inoue R, et al. The influence of long-term use of proton pump inhibitors on the gut microbiota: an age-sex-matched case-control study. J Clin Biochem Nutr. 2018;62:100–5.
doi: 10.3164/jcbn.17-78
pubmed: 29371761
Caporaso JG, Kuczynski J, Stombaugh J, Bittinger K, Bushman FD, Costello EK, et al. QIIME allows analysis of high-throughput community sequencing data. Nat Methods. 2010;7:335–6.
doi: 10.1038/nmeth.f.303
pubmed: 20383131
pmcid: 3156573
Edgar RC. Search and clustering orders of magnitude faster than BLAST. Bioinformatics (Oxford, England). 2010;26:2460–1.
doi: 10.1093/bioinformatics/btq461
Edgar RC, Haas BJ, Clemente JC, et al. UCHIME improves sensitivity and speed of chimera detection. Bioinformatics (Oxford, England). 2011;27:2194–200.
doi: 10.1093/bioinformatics/btr381
Koliada A, Syzenko G, Moseiko V, et al. Association between body mass index and Firmicutes/Bacteroidetes ratio in an adult Ukrainian population. BMC Microbiol. 2017;17:120.
doi: 10.1186/s12866-017-1027-1
pubmed: 28532414
pmcid: 5440985
Ley RE, Backhed F, Turnbaugh P, et al. Obesity alters gut microbial ecology. Proc Natl Acad Sci USA. 2005;102:11070–5.
doi: 10.1073/pnas.0504978102
pubmed: 16033867
Mathur R, Barlow GM. Obesity and the microbiome. Expert Rev Gastroenterol Hepatol. 2015;9:1087–99.
doi: 10.1586/17474124.2015.1051029
pubmed: 26082274
Ignacio A, Fernandes MR, Rodrigues VA, et al. Correlation between body mass index and faecal microbiota from children. Clin Microbiol Infect. 2016;22:258.e1–e8.
doi: 10.1016/j.cmi.2015.10.031
Schwiertz A, Taras D, Schafer K, et al. Microbiota and SCFA in lean and overweight healthy subjects. Obesity (Silver Spring, Md). 2010;18:190–5.
doi: 10.1038/oby.2009.167
Odamaki T, Kato K, Sugahara H, et al. Age-related changes in gut microbiota composition from newborn to centenarian: a cross-sectional study. BMC Microbiol. 2016;16:90.
doi: 10.1186/s12866-016-0708-5
pubmed: 27220822
pmcid: 4879732
Wang JJ, Wang J, Pang XY, et al. Sex differences in colonization of gut microbiota from a man with short-term vegetarian and inulin-supplemented diet in germ-free mice. Sci Rep. 2016;6:36137.
doi: 10.1038/srep36137
pubmed: 27796317
pmcid: 5086848
Markle JG, Frank DN, Mortin-Toth S, et al. Sex differences in the gut microbiome drive hormone-dependent regulation of autoimmunity. Science. 2013;339:1084–8.
doi: 10.1126/science.1233521
pubmed: 23328391
Org E, Mehrabian M, Parks BW, et al. Sex differences and hormonal effects on gut microbiota composition in mice. Gut Microbes. 2016;7:313–22.
doi: 10.1080/19490976.2016.1203502
pubmed: 27355107
pmcid: 4988450
Fransen F, van Beek AA, Borghuis T, et al. The impact of gut microbiota on gender-specific differences in immunity. Front Immunol. 2017;8:754.
doi: 10.3389/fimmu.2017.00754
pubmed: 28713378
pmcid: 5491612
Yatsunenko T, Rey FE, Manary MJ, et al. Human gut microbiome viewed across age and geography. Nature. 2012;486:222–7.
doi: 10.1038/nature11053
pubmed: 22699611
pmcid: 3376388
Sonnenburg JL, Backhed F. Diet-microbiota interactions as moderators of human metabolism. Nature. 2016;535:56–64.
doi: 10.1038/nature18846
pubmed: 27383980
pmcid: 5991619
Kasai C, Sugimoto K, Moritani I, et al. Comparison of the gut microbiota composition between obese and non-obese individuals in a Japanese population, as analyzed by terminal restriction fragment length polymorphism and next-generation sequencing. BMC Gastroenterol. 2015;15:100.
doi: 10.1186/s12876-015-0330-2
pubmed: 26261039
pmcid: 4531509
Lewis SJ, Heaton KW. Stool form scale as a useful guide to intestinal transit time. Scand J Gastroenterol. 1997;32:920–4.
doi: 10.3109/00365529709011203
pubmed: 9299672
Tian H, Ge X, Nie Y, et al. Fecal microbiota transplantation in patients with slow-transit constipation: a randomized, clinical trial. PLoS One. 2017;12:e0171308.
doi: 10.1371/journal.pone.0171308
pubmed: 28158276
pmcid: 5291446
Halkjaer SI, Boolsen AW, Gunther S, et al. Can fecal microbiota transplantation cure irritable bowel syndrome? World J Gastroenterol. 2017;23:4112–20.
doi: 10.3748/wjg.v23.i22.4112
pubmed: 28652664
pmcid: 5473130
Abadi ATB. Fecal microbiota transplantation against irritable bowel syndrome? Rigorous randomized clinical trials are required. World J Gastrointest Pharmacol Ther. 2017;8:208–9.
doi: 10.4292/wjgpt.v8.i4.208
pubmed: 29152407
pmcid: 5680168
Mizuno S, Masaoka T, Naganuma M, et al. Bifidobacterium-rich fecal donor may be a positive predictor for successful fecal microbiota transplantation in patients with irritable bowel syndrome. Digestion. 2017;96:29–38.
doi: 10.1159/000471919
pubmed: 28628918
pmcid: 5637308
Agrawal A, Houghton LA, Morris J, et al. Clinical trial: the effects of a fermented milk product containing Bifidobacterium lactis DN-173 010 on abdominal distension and gastrointestinal transit in irritable bowel syndrome with constipation. Aliment Pharmacol Ther. 2009;29:104–14.
doi: 10.1111/j.1365-2036.2008.03853.x
pubmed: 18801055
Kim HJ, Vazquez Roque MI, Camilleri M, et al. A randomized controlled trial of a probiotic combination VSL# 3 and placebo in irritable bowel syndrome with bloating. Neurogastroenterol Motil. 2005;17:687–96.
doi: 10.1111/j.1365-2982.2005.00695.x
pubmed: 16185307