Label-free quantitative proteomics analysis reveals distinct molecular characteristics in endocardial endothelium.
Aortic endothelial cells
Bioinformatics
Endocardial endothelium
Label-free quantitative proteomics
Transcription Factors
Journal
Molecular and cellular biochemistry
ISSN: 1573-4919
Titre abrégé: Mol Cell Biochem
Pays: Netherlands
ID NLM: 0364456
Informations de publication
Date de publication:
Jan 2019
Jan 2019
Historique:
received:
30
11
2017
accepted:
16
06
2018
pubmed:
24
6
2018
medline:
7
3
2019
entrez:
24
6
2018
Statut:
ppublish
Résumé
Endocardial endothelium, which lines the chambers of the heart, is distinct in its origin, structure, and function. Characterization studies using genomics and proteomics have reported molecular signatures supporting the structural and functional heterogeneity of various endothelial cells. However, though functionally very important, no studies at protein level have been conducted so far characterizing endocardial endothelium. In this study, we used endothelial cells from pig heart to investigate if endocardial endothelial cells are distinct at the proteome level. Using a high-throughput liquid chromatography-tandem mass spectrometry for proteome profiling and expression, we identified sets of proteins that belong to specific biological processes and metabolic pathways in endocardial endothelial cells supporting its specific structural and functional roles. The study also identified several transcription factors and cell surface markers, which may have roles in the specificity of endocardial endothelium. The detection of sets proteins preferentially expressed in endocardial endothelium offers new insights into its role in the regulation of cardiac function. Data are made available through ProteomeXchange with identifier PXD009194.
Identifiants
pubmed: 29934862
doi: 10.1007/s11010-018-3387-8
pii: 10.1007/s11010-018-3387-8
doi:
Substances chimiques
Biomarkers
0
Proteome
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
1-10Références
Vasc Health Risk Manag. 2013;9:195-206
pubmed: 23662065
J Am Heart Assoc. 2016 Apr 18;5(4):e003170
pubmed: 27091183
Cardiovasc Res. 1998 May;38(2):281-90
pubmed: 9709389
Semin Thromb Hemost. 2015 Sep;41(6):549-55
pubmed: 26270112
Br J Pharmacol. 1993 Apr;108(4):1107-10
pubmed: 7683563
Nat Protoc. 2011 Jun;6(6):743-60
pubmed: 21637195
PLoS One. 2015 Nov 13;10(11):e0142940
pubmed: 26565707
J Biomech. 2017 Jan 4;50:11-19
pubmed: 27916240
Proc Natl Acad Sci U S A. 2002 Oct 1;99(20):13108-13
pubmed: 12228731
Arterioscler Thromb Vasc Biol. 1997 Jul;17(7):1193-202
pubmed: 9261246
Proteomics. 2003 May;3(5):714-23
pubmed: 12748950
Biochim Biophys Acta. 2016 Jul;1863(7 Pt B):1937-47
pubmed: 26828773
Dev Biol. 2009 Sep 1;333(1):78-89
pubmed: 19576203
Physiol Rev. 2003 Jan;83(1):59-115
pubmed: 12506127
Biomed Res Int. 2014;2014:923805
pubmed: 24745027
BMC Genomics. 2011 Dec 22;12:630
pubmed: 22192797
Sci Rep. 2017 Aug 17;7(1):8588
pubmed: 28819266
Blood. 2007 Jan 15;109(2):613-5
pubmed: 16990601
Acta Physiol (Oxf). 2009 Feb;195(2):231-45
pubmed: 18826501
Nucleic Acids Res. 2016 Jan 4;44(D1):D481-7
pubmed: 26656494
Circ Res. 1988 Feb;62(2):358-66
pubmed: 3338120
Proc Natl Acad Sci U S A. 2005 Oct 25;102(43):15545-50
pubmed: 16199517
Rom J Morphol Embryol. 2012;53(1):135-42
pubmed: 22395512
Mol Cell Biochem. 2003 Nov;253(1-2):113-23
pubmed: 14619961
Bioinformatics. 2009 Feb 15;25(4):555-6
pubmed: 19129209
Cell. 2008 Dec 12;135(6):1053-64
pubmed: 19070576
Clin Exp Nephrol. 2013 Jun;17(3):327-37
pubmed: 23229650
Cell Tissue Res. 2009 Jan;335(1):67-73
pubmed: 18682987
Cell. 2012 Nov 21;151(5):1083-96
pubmed: 23178125
Proc Natl Acad Sci U S A. 2007 Nov 6;104(45):17692-7
pubmed: 17962413
Am J Physiol. 1999 May;276(5):H1755-68
pubmed: 10330261