Platelet-rich fibrin/biphasic calcium phosphate impairs osteoclast differentiation and promotes apoptosis by the intrinsic mitochondrial pathway in chronic periodontitis.
apoptosis
biomaterials
bone regeneration
osteoclasts
periodontitis
Journal
Journal of periodontology
ISSN: 1943-3670
Titre abrégé: J Periodontol
Pays: United States
ID NLM: 8000345
Informations de publication
Date de publication:
01 2019
01 2019
Historique:
received:
13
05
2017
revised:
08
09
2017
accepted:
10
02
2018
pubmed:
30
6
2018
medline:
27
11
2019
entrez:
30
6
2018
Statut:
ppublish
Résumé
The study explored the effect of platelet-rich fibrin/biphasic calcium phosphate (PRF/BCP) on differentiation and survival of osteoclasts obtained from peripheral blood of CP patients. Peripheral blood mononuclear cells (PBMCs) from 25 patients with chronic periodontitis (CP) and 25 healthy individuals were assayed for cluster of differentiation14 We observed a relative increase in the proportion of circulating osteoclasts in test group than control group (healthy individuals). In addition, osteoclast precursors in untreated cells (CP) were more osteoclastogenic as compared to cells treated with PRF/BCP and hence, there was a significant increase in the number of osteoclasts in CP. In PRF/BCP treated cells, we found a direct inhibition of transcription factor NF-κB with an increased caspase 3/9 levels and caspase 3 activity. Additionally, the protein expression and transcriptional profile of Bax was upregulated and Bcl-2 and Bcl-xL levels were down-regulated on treatment with PRF/BCP. Our results revealed that the PRF/BCP displayed an inhibitory role in osteoclasts formation and its molecular mechanism of action was related to the apoptosis induction through intrinsic mitochondrial pathway.
Sections du résumé
BACKGROUND
The study explored the effect of platelet-rich fibrin/biphasic calcium phosphate (PRF/BCP) on differentiation and survival of osteoclasts obtained from peripheral blood of CP patients.
METHODS
Peripheral blood mononuclear cells (PBMCs) from 25 patients with chronic periodontitis (CP) and 25 healthy individuals were assayed for cluster of differentiation14
RESULTS
We observed a relative increase in the proportion of circulating osteoclasts in test group than control group (healthy individuals). In addition, osteoclast precursors in untreated cells (CP) were more osteoclastogenic as compared to cells treated with PRF/BCP and hence, there was a significant increase in the number of osteoclasts in CP. In PRF/BCP treated cells, we found a direct inhibition of transcription factor NF-κB with an increased caspase 3/9 levels and caspase 3 activity. Additionally, the protein expression and transcriptional profile of Bax was upregulated and Bcl-2 and Bcl-xL levels were down-regulated on treatment with PRF/BCP.
CONCLUSION
Our results revealed that the PRF/BCP displayed an inhibitory role in osteoclasts formation and its molecular mechanism of action was related to the apoptosis induction through intrinsic mitochondrial pathway.
Identifiants
pubmed: 29958327
doi: 10.1002/JPER.17-0306
doi:
Substances chimiques
Hydroxyapatites
0
RANK Ligand
0
hydroxyapatite-beta tricalcium phosphate
0
Macrophage Colony-Stimulating Factor
81627-83-0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
61-71Informations de copyright
© 2018 American Academy of Periodontology.