Enhancing the Secretion of a Glyco-Engineered Anti-CD20 scFv-Fc Antibody in Hairy Root Cultures.
Antibodies, Monoclonal
/ genetics
Antigens, CD20
/ genetics
Fucose
/ genetics
Glycosylation
Humans
Luminescent Proteins
/ genetics
Plant Roots
/ genetics
Plants, Genetically Modified
/ genetics
Protein Engineering
/ methods
Recombinant Proteins
/ genetics
Single-Chain Antibodies
/ genetics
Nicotiana
/ genetics
Xylose
/ genetics
Red Fluorescent Protein
N-glycosylation
auxins
hairy roots
molecular farming
non-Hodgkin lymphoma
scFv-Fc antibody
Journal
Biotechnology journal
ISSN: 1860-7314
Titre abrégé: Biotechnol J
Pays: Germany
ID NLM: 101265833
Informations de publication
Date de publication:
Mar 2019
Mar 2019
Historique:
received:
06
02
2018
revised:
26
06
2018
pubmed:
6
7
2018
medline:
15
6
2019
entrez:
6
7
2018
Statut:
ppublish
Résumé
Hairy root (HR) cultures represent an attractive platform for the production of heterologous proteins, due to the possibility of secreting the molecule of interest in the culture medium. The main limitation is the low accumulation yields of heterologous proteins. The aim of this study is to enhance the accumulation of a tumor-targeting antibody with a human-compatible glycosylation profile in HR culture medium. To this aim, the authors produce Nicotiana benthamiana HR cultures expressing the red fluorescent protein (RFP) to easily screen for different auxins able to induce heterologous protein secretion in the medium. The hormone 2,4-dichlorophenoxyacetic acid (2,4-D) is found to induce high accumulation levels (334 mg L
Identifiants
pubmed: 29975457
doi: 10.1002/biot.201800081
doi:
Substances chimiques
Antibodies, Monoclonal
0
Antigens, CD20
0
Luminescent Proteins
0
Recombinant Proteins
0
Single-Chain Antibodies
0
Fucose
28RYY2IV3F
Xylose
A1TA934AKO
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
e1800081Subventions
Organisme : Italian Ministry of Foreign Affairs - Direzione Generale per la Promozione del Sistema Paese - Unità per la cooperazione scientifica e tecnologica bilaterale e multilaterale
Informations de copyright
© 2018 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.