Nanoparticle-induced enzyme pretreatment method for increased glucose production from lignocellulosic biomass under cold conditions.
enzyme pretreatment
lignocellulose processing
Journal
Journal of the science of food and agriculture
ISSN: 1097-0010
Titre abrégé: J Sci Food Agric
Pays: England
ID NLM: 0376334
Informations de publication
Date de publication:
30 Jan 2019
30 Jan 2019
Historique:
received:
09
03
2018
revised:
04
07
2018
accepted:
05
07
2018
pubmed:
13
7
2018
medline:
12
1
2019
entrez:
13
7
2018
Statut:
ppublish
Résumé
Appropriate pretreatment strategies that fractionate sugarcane bagasse (SB) are essential for the successful use of this feedstock in ethanol production. In this paper, we investigate a purely enzymatic process to achieve increased production of reducing sugars (RS) from SB in the presence of MgO nanoparticles (MgN) subjected to a three-step sequential enzyme treatment. Pretreatment of SB with protease activated by magnesium oxide nanoparticles (MgN-pro) at 95 °C showed an increase in amino acid production by 6.18-fold compared to the untreated enzyme set at the same temperature. High-performance liquid chromatography (HPLC) studies showed an 18-fold removal of lignin from the samples subjected to protease (+ MgN) treatment compared to untreated samples. When the MgN-pro pretreated samples were subjected to pretreatment with xylanase activated by magnesium oxide nanoparticles (MgN-xyl), more than 30-fold increased RS was produced at 8 °C compared to cellulase (cel) pretreated samples. Xylanase pretreated SB samples produced 1.82- and 1.91-fold increased reducing sugar and glucose respectively at 8 °C in the presence of MgN compared to untreated samples at the same temperature. The results presented here show the efficiency of the proposed method for improving the enzymatic digestibility of SB and explain the pretreatment action mechanism. These findings have potential implications in bio-ethanol, bio-fuel, and agro industries. © 2018 Society of Chemical Industry.
Sections du résumé
BACKGROUND
BACKGROUND
Appropriate pretreatment strategies that fractionate sugarcane bagasse (SB) are essential for the successful use of this feedstock in ethanol production. In this paper, we investigate a purely enzymatic process to achieve increased production of reducing sugars (RS) from SB in the presence of MgO nanoparticles (MgN) subjected to a three-step sequential enzyme treatment.
RESULT
RESULTS
Pretreatment of SB with protease activated by magnesium oxide nanoparticles (MgN-pro) at 95 °C showed an increase in amino acid production by 6.18-fold compared to the untreated enzyme set at the same temperature. High-performance liquid chromatography (HPLC) studies showed an 18-fold removal of lignin from the samples subjected to protease (+ MgN) treatment compared to untreated samples. When the MgN-pro pretreated samples were subjected to pretreatment with xylanase activated by magnesium oxide nanoparticles (MgN-xyl), more than 30-fold increased RS was produced at 8 °C compared to cellulase (cel) pretreated samples. Xylanase pretreated SB samples produced 1.82- and 1.91-fold increased reducing sugar and glucose respectively at 8 °C in the presence of MgN compared to untreated samples at the same temperature.
CONCLUSION
CONCLUSIONS
The results presented here show the efficiency of the proposed method for improving the enzymatic digestibility of SB and explain the pretreatment action mechanism. These findings have potential implications in bio-ethanol, bio-fuel, and agro industries. © 2018 Society of Chemical Industry.
Substances chimiques
Biofuels
0
lignocellulose
11132-73-3
Magnesium Oxide
3A3U0GI71G
Ethanol
3K9958V90M
Cellulose
9004-34-6
Lignin
9005-53-2
bagasse
9006-97-7
Cellulase
EC 3.2.1.4
Peptide Hydrolases
EC 3.4.-
Glucose
IY9XDZ35W2
Types de publication
Evaluation Study
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
767-780Informations de copyright
© 2018 Society of Chemical Industry.