Initial cell plating density affects properties of human primary synovial mesenchymal stem cells.
chondrogenesis
mesenchymal stem cell
plating density
primary cell
synovium
Journal
Journal of orthopaedic research : official publication of the Orthopaedic Research Society
ISSN: 1554-527X
Titre abrégé: J Orthop Res
Pays: United States
ID NLM: 8404726
Informations de publication
Date de publication:
06 2019
06 2019
Historique:
received:
15
02
2018
accepted:
01
07
2018
pubmed:
24
7
2018
medline:
21
12
2019
entrez:
24
7
2018
Statut:
ppublish
Résumé
Synovial mesenchymal stem cells (MSCs) appear to be an attractive cell source in cartilage and meniscus regeneration because of their high proliferative and chondrogenic potentials. Two methods are used to culture synovial nucleated cells in the preparation of primary synovial MSCs. In one method, the cells are plated at low density to make cell colonies. In the other method, the cells are plated at high density. We investigated the effects of initial cell density on proliferation, surface markers, and multipotentiality, including chondrogenesis in primary synovial MSCs. Human synovium was obtained from the knee joints of patients with osteoarthritis after total knee arthroplasty. Immediately after enzyme digestion, the synovial nucleated cells were plated in densities of 10
Identifiants
pubmed: 30035340
doi: 10.1002/jor.24112
pmc: PMC6585790
doi:
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
1358-1367Informations de copyright
© 2018 The Authors. Journal of Orthopaedic Research® Published by Wiley Periodicals, Inc.
Références
Arthritis Rheum. 2008 Dec;58(12):3809-19
pubmed: 19035511
J Bone Miner Res. 2007 Oct;22(10):1592-602
pubmed: 17605633
Anticancer Res. 2011 Jun;31(6):2283-90
pubmed: 21737653
Exp Hematol. 1976 Sep;4(5):267-74
pubmed: 976387
Arthritis Rheum. 2006 Mar;54(3):843-53
pubmed: 16508965
Science. 1997 Apr 4;276(5309):71-4
pubmed: 9082988
Hum Genet. 1995 Dec;96(6):651-4
pubmed: 8522321
Genes Chromosomes Cancer. 1993 Apr;6(4):232-4
pubmed: 7685626
Cell Tissue Res. 2008 Aug;333(2):207-15
pubmed: 18560897
Mediators Inflamm. 2014;2014:702057
pubmed: 25548436
Stem Cell Res Ther. 2016 Aug 31;7(1):125
pubmed: 27581859
Osteoarthritis Cartilage. 2015 Jun;23(6):1007-17
pubmed: 25683149
Clin Orthop Relat Res. 2015 Jul;473(7):2316-26
pubmed: 25925939
Blood. 1998 Dec 15;92(12):4677-90
pubmed: 9845534
J Cell Biochem. 2006 Jan 1;97(1):84-97
pubmed: 16088956
Stem Cells. 2002;20(6):530-41
pubmed: 12456961
J Bone Miner Res. 2004 Feb;19(2):256-64
pubmed: 14969395
J Cell Biol. 2007 Apr 9;177(1):73-86
pubmed: 17420291
Cell Stem Cell. 2011 Aug 5;9(2):97-102
pubmed: 21816361
Cell Transplant. 2008;17(5):549-57
pubmed: 18714674
Proc Natl Acad Sci U S A. 2002 Apr 2;99(7):4397-402
pubmed: 11917104
Arthritis Rheum. 2005 Aug;52(8):2521-9
pubmed: 16052568
Cytotherapy. 2006;8(4):315-7
pubmed: 16923606