Determination of phosphatidylethanol 16:0/18:1 in whole blood by 96-well supported liquid extraction and UHPLC-MS/MS.

Alcohol Drinking Chromatography, High Pressure Liquid / methods Glycerophospholipids / blood High-Throughput Screening Assays Humans Limit of Detection Liquid-Liquid Extraction / methods Reproducibility of Results Tandem Mass Spectrometry / methods
96-well supported liquid extraction Alcohol LC-MS/MS PEth 16:0/18:1 phosphatidylethanol

Journal

Journal of clinical laboratory analysis
ISSN: 1098-2825
Titre abrégé: J Clin Lab Anal
Pays: United States
ID NLM: 8801384

Informations de publication

Date de publication:
Jan 2019
Historique:
revised: 20 06 2018
received: 25 04 2018
accepted: 05 07 2018
pubmed: 27 7 2018
medline: 16 2 2019
entrez: 27 7 2018
Statut: ppublish

Résumé

Phosphatidylethanols (PEths) are specific, direct alcohol biomarkers that can be determined in human blood to distinguish between heavy and social drinking. PEth 16:0/18:1 is among the most predominant PEth homologues in human blood. The aim of the study was to develop a high throughput and sensitive UHPLC-MS/MS method for the determination of PEth 16:0/18:1 in whole blood. Whole blood samples were prepared by 96-well supported liquid extraction (SLE). Extracted samples were analyzed for PEth 16:0/18:1 by reversed phase UHPLC-MS/MS. The developed UHPLC-MS/MS method was fully validated in whole blood with PEth 16:0/18:1-D For the first time, 96-well SLE was used for preparation of a PEth homologue in biological samples. A mixture of tert-butyl methyl ether and 2-propanol (5:1, v:v) was chosen as organic eluent based on an evaluation of extraction recovery, purity of extracts, and evaporation time. The developed UHPLC-MS/MS method can be used for high throughput analyses and sensitive determinations of PEth 16:0/18:1 in whole blood.

Sections du résumé

BACKGROUND BACKGROUND
Phosphatidylethanols (PEths) are specific, direct alcohol biomarkers that can be determined in human blood to distinguish between heavy and social drinking. PEth 16:0/18:1 is among the most predominant PEth homologues in human blood. The aim of the study was to develop a high throughput and sensitive UHPLC-MS/MS method for the determination of PEth 16:0/18:1 in whole blood.
METHODS METHODS
Whole blood samples were prepared by 96-well supported liquid extraction (SLE). Extracted samples were analyzed for PEth 16:0/18:1 by reversed phase UHPLC-MS/MS.
RESULTS RESULTS
The developed UHPLC-MS/MS method was fully validated in whole blood with PEth 16:0/18:1-D
CONCLUSION CONCLUSIONS
For the first time, 96-well SLE was used for preparation of a PEth homologue in biological samples. A mixture of tert-butyl methyl ether and 2-propanol (5:1, v:v) was chosen as organic eluent based on an evaluation of extraction recovery, purity of extracts, and evaporation time. The developed UHPLC-MS/MS method can be used for high throughput analyses and sensitive determinations of PEth 16:0/18:1 in whole blood.

Identifiants

DOI: 10.1002/jcla.22631 PMID: 30047172 PMC: PMC6430335
pubmed: 30047172
doi: 10.1002/jcla.22631
pmc: PMC6430335
doi:

Substances chimiques

Glycerophospholipids 0
phosphatidylethanol 0

Types de publication

Journal Article

Langues

eng

Sous-ensembles de citation

IM

Pagination

e22631

Informations de copyright

© 2018 Wiley Periodicals, Inc.

Références

Anal Bioanal Chem. 2011 Sep;401(4):1163-6
pubmed: 21743983
Alcohol. 2018 Mar;67:7-13
pubmed: 29304397
Bioanalysis. 2013 Feb;5(3):285-8
pubmed: 23394695
Forensic Sci Int. 2007 Jan 17;165(2-3):216-24
pubmed: 16781833
Bioanalysis. 2014 Sep;6(18):2481-514
pubmed: 25384597
Sci Justice. 2010 Dec;50(4):166-76
pubmed: 21075293
J Chromatogr B Analyt Technol Biomed Life Sci. 2012 Feb 1;883-884:177-88
pubmed: 22119506
Bioanalysis. 2012 Mar;4(6):651-60
pubmed: 22452256
J Pharm Biomed Anal. 2016 Sep 5;128:426-437
pubmed: 27344632
Rapid Commun Mass Spectrom. 2005;19(3):401-7
pubmed: 15645520
J Biol Chem. 1992 Aug 25;267(24):16834-40
pubmed: 1512226
Drug Test Anal. 2011 Apr;3(4):195-200
pubmed: 21438164
Alcohol Clin Exp Res. 2014 Apr;38(4):1078-85
pubmed: 24511895
Clin Biochem Rev. 2008 Aug;29 Suppl 1:S49-52
pubmed: 18852857
J Chromatogr A. 2011 Dec 30;1218(52):9366-74
pubmed: 22119139
J Chromatogr B Analyt Technol Biomed Life Sci. 2012 Apr 1;891-892:71-80
pubmed: 22410088
J Chromatogr B Analyt Technol Biomed Life Sci. 2014 Nov 15;971:72-80
pubmed: 25264915
J Chromatogr B Biomed Sci Appl. 1998 Feb 13;705(2):243-9
pubmed: 9521560
J Pharm Biomed Anal. 2010 Nov 2;53(3):228-34
pubmed: 20236782
J Pharm Biomed Anal. 2012 Jan 25;58:71-7
pubmed: 21993197
Prog Lipid Res. 2008 Sep;47(5):348-80
pubmed: 18435934
BMC Biochem. 2005 Sep 27;6:18
pubmed: 16188025
J Clin Lab Anal. 2019 Jan;33(1):e22631
pubmed: 30047172
J Chromatogr A. 2017 Oct 13;1519:1-8
pubmed: 28890270
Clin Chim Acta. 2018 Apr;479:38-42
pubmed: 29309773
Forensic Sci Int. 2010 Jul 15;200(1-3):1-20
pubmed: 20304569
Anal Bioanal Chem. 2010 Apr;396(7):2415-23
pubmed: 20127079
Bioanalysis. 2012 Feb;4(3):305-20
pubmed: 22303834
J Chromatogr A. 2011 Nov 18;1218(46):8423-31
pubmed: 21999914
Clin Chim Acta. 1978 Dec 15;90(3):203-8
pubmed: 31991
Tidsskr Nor Laegeforen. 2016 Oct 25;136(19):1643-1647
pubmed: 27790892
Biochem Biophys Res Commun. 1987 Feb 13;142(3):958-63
pubmed: 3827907
Biochim Biophys Acta. 1984 Mar 27;793(1):119-22
pubmed: 6704410
Int J Legal Med. 2013 May;127(3):603-10
pubmed: 23208617
Anal Bioanal Chem. 2016 Jan;408(3):825-38
pubmed: 26597914
Bioanalysis. 2015;7(17):2177-86
pubmed: 26378936
J Chromatogr A. 2014 May 30;1344:83-90
pubmed: 24780257
Bioanalysis. 2016 Feb;8(3):229-51
pubmed: 26795230
Clin Chem. 2009 Jul;55(7):1395-405
pubmed: 19423735
Anal Chim Acta. 2017 Apr 22;963:68-75
pubmed: 28335977
Clin Chim Acta. 2011 Jul 15;412(15-16):1428-35
pubmed: 21531215
J Chromatogr A. 2011 Jan 14;1218(2):359-61
pubmed: 21159347
J Anal Toxicol. 2018 Jan 1;42(1):33-41
pubmed: 28977407
Alcohol Clin Exp Res. 2016 Nov;40(11):2292-2295
pubmed: 27716960
J Chromatogr B Analyt Technol Biomed Life Sci. 2013 May 15;927:112-23
pubmed: 23380540
FEBS Lett. 1983 Feb 7;152(1):24-8
pubmed: 6840274
J Anal Toxicol. 2013 Sep;37(7):452-74
pubmed: 23934984
Analyst. 2014 May 21;139(10):2265-76
pubmed: 24633191
Drug Test Anal. 2017 May;9(5):808-823
pubmed: 27464485
Clin Chem. 1994 Jul;40(7 Pt 1):1233-8
pubmed: 8013092
Forensic Sci. 1976 Mar-Apr;7(2):131-40
pubmed: 964805
J Anal Toxicol. 2011 Jun;35(5):280-93
pubmed: 21619723
Anal Chem. 2003 Jul 1;75(13):3019-30
pubmed: 12964746

Auteurs

Thomas Berg (T)

Section of Drug Abuse Research, Department of Forensic Sciences, Division of Laboratory Medicine, Oslo University Hospital, Oslo, Norway.
ORCID: 0000-0002-7675-2095

Elin Eliassen (E)

Section of Drug Abuse Research, Department of Forensic Sciences, Division of Laboratory Medicine, Oslo University Hospital, Oslo, Norway.

Benedicte Jørgenrud (B)

Section of Drug Abuse Research, Department of Forensic Sciences, Division of Laboratory Medicine, Oslo University Hospital, Oslo, Norway.

Saranda Kabashi (S)

Section of Drug Abuse Research, Department of Forensic Sciences, Division of Laboratory Medicine, Oslo University Hospital, Oslo, Norway.

Alexey Petukhov (A)

Moscow Scientific Practical Center of Drug Addiction, Moscow Municipal Department of Healthcare, Moscow, Russia.
I.M. Sechenov First Moscow State Medical University, Moscow, Russia.

Stig Tore Bogstrand (ST)

Section of Drug Abuse Research, Department of Forensic Sciences, Division of Laboratory Medicine, Oslo University Hospital, Oslo, Norway.
Lovisenberg Diaconal University College, Oslo, Norway.

Articles similaires

[Redispensing of expensive oral anticancer medicines: a practical application].

Lisanne N van Merendonk, Kübra Akgöl, Bastiaan Nuijen
1.00
Humans Antineoplastic Agents Administration, Oral Drug Costs Counterfeit Drugs

Smoking Cessation and Incident Cardiovascular Disease.

Jun Hwan Cho, Seung Yong Shin, Hoseob Kim et al.
1.00
Humans Male Smoking Cessation Cardiovascular Diseases Female

Evaluation of Low-Value Services Across Major Medicare Advantage Insurers and Traditional Medicare.

Ciara Duggan, Adam L Beckman, Ishani Ganguli et al.
1.00
Humans United States Aged Cross-Sectional Studies Medicare Part C

Effectiveness of Virtual Yoga for Chronic Low Back Pain: A Randomized Clinical Trial.

Hallie Tankha, Devyn Gaskins, Amanda Shallcross et al.
1.00
Humans Yoga Low Back Pain Female Male

Classifications MeSH

questionsmedicales.fr › Comportement et mécanismes comportementaux › Comportement › Comportement dipsique › Consommation d'alcool › Determination of phosphatidylethanol 16:0/18:1...
questionsmedicales.fr › Techniques d'investigation › Techniques de chimie analytique › Chromatographie › Chromatographie en phase liquide › Chromatographie en phase liquide à haute performance › Determination of phosphatidylethanol 16:0/18:1...
questionsmedicales.fr › Lipides › Lipides membranaires › Phospholipides › Glycérophosphate › Acides phosphatidiques › Glycérophospholipides › Determination of phosphatidylethanol 16:0/18:1...
questionsmedicales.fr › Techniques d'investigation › Tests de criblage à haut débit › Determination of phosphatidylethanol 16:0/18:1...
questionsmedicales.fr › Eucaryotes › Animaux › Chordés › Vertébrés › Mammifères › Eutheria › Primates › Haplorhini › Catarrhini › Hominidae › Humains › Determination of phosphatidylethanol 16:0/18:1...
questionsmedicales.fr › Environnement et santé publique › Santé publique › Méthodes épidémiologiques › Statistiques comme sujet › Sensibilité et spécificité › Limite de détection › Determination of phosphatidylethanol 16:0/18:1...
questionsmedicales.fr › Techniques d'investigation › Techniques de chimie analytique › Fractionnement chimique › Extraction liquide-liquide › Determination of phosphatidylethanol 16:0/18:1...
questionsmedicales.fr › Environnement et santé publique › Santé publique › Méthodes épidémiologiques › Méthodologie en recherche épidémiologique › Reproductibilité des résultats › Determination of phosphatidylethanol 16:0/18:1...
questionsmedicales.fr › Techniques d'investigation › Techniques de chimie analytique › Spectrométrie de masse › Spectrométrie de masse en tandem › Determination of phosphatidylethanol 16:0/18:1...