Patients with a fast progression profile in geographic atrophy have increased CD200 expression on circulating monocytes.
Aged
Aged, 80 and over
Antigens, CD
/ biosynthesis
Biomarkers
/ blood
Case-Control Studies
Disease Progression
Female
Flow Cytometry
Fluorescein Angiography
/ methods
Follow-Up Studies
Fundus Oculi
Geographic Atrophy
/ blood
Humans
Male
Monocytes
/ metabolism
Prospective Studies
Retina
/ pathology
Tomography, Optical Coherence
/ methods
CD200 membrane glycoprotein
age-related macular degeneration
geographic atrophy
microglia
monocytes
Journal
Clinical & experimental ophthalmology
ISSN: 1442-9071
Titre abrégé: Clin Exp Ophthalmol
Pays: Australia
ID NLM: 100896531
Informations de publication
Date de publication:
01 2019
01 2019
Historique:
received:
16
05
2018
revised:
27
06
2018
accepted:
13
07
2018
pubmed:
27
7
2018
medline:
11
2
2020
entrez:
27
7
2018
Statut:
ppublish
Résumé
Geographic atrophy (GA) is a progressing atrophy of the neuroretina with no treatment option. Age-related malfunction of retinal microglia amplifies response towards age-related tissue stress in age-related macular degeneration. Here, we investigated monocyte CD200 expression - the circulating middleman negotiating retinal microglial activity - in a poorly understood subtype of age-related macular degeneration. Prospective case-control study. Forty-six patients with GA and 26 healthy controls were included. All participants were subjected to a structured interview and detailed retinal examination. Controls were recruited from patient's spouses accompanying them in the clinic to match the groups best possibly. Participants had no history of immune disorders or cancer, and did not receive any immune-modulating medication. Patients did not have any history or sign of choroidal neovascularization in either eye. Fresh drawn blood was stained with monoclonal antibodies and prepared for flow cytometry to evaluate CD200 expression in monocytes and their functional subsets. The percentage of CD200+ monocytes in patients and controls. We found that monocytes were more CD200 positive in patients with GA compared to healthy age-matched controls. Then, we explored the potential relationship between CD200 expression and important fundus autofluorescence patterns that predict disease progression. Patients with a high risk of progression (patients with high degree of hyperautofluorescence) had distinctly increased CD200 expression compared to other patients with GA. Our data reveals that abnormal monocytic CD200 expression is present in GA, and in particular among those identified as fast progressors.
Sections du résumé
IMPORTANCE
Geographic atrophy (GA) is a progressing atrophy of the neuroretina with no treatment option.
BACKGROUND
Age-related malfunction of retinal microglia amplifies response towards age-related tissue stress in age-related macular degeneration. Here, we investigated monocyte CD200 expression - the circulating middleman negotiating retinal microglial activity - in a poorly understood subtype of age-related macular degeneration.
DESIGN
Prospective case-control study.
PARTICIPANTS
Forty-six patients with GA and 26 healthy controls were included.
METHODS
All participants were subjected to a structured interview and detailed retinal examination. Controls were recruited from patient's spouses accompanying them in the clinic to match the groups best possibly. Participants had no history of immune disorders or cancer, and did not receive any immune-modulating medication. Patients did not have any history or sign of choroidal neovascularization in either eye. Fresh drawn blood was stained with monoclonal antibodies and prepared for flow cytometry to evaluate CD200 expression in monocytes and their functional subsets.
MAIN OUTCOME MEASURES
The percentage of CD200+ monocytes in patients and controls.
RESULTS
We found that monocytes were more CD200 positive in patients with GA compared to healthy age-matched controls. Then, we explored the potential relationship between CD200 expression and important fundus autofluorescence patterns that predict disease progression. Patients with a high risk of progression (patients with high degree of hyperautofluorescence) had distinctly increased CD200 expression compared to other patients with GA.
CONCLUSIONS AND RELEVANCE
Our data reveals that abnormal monocytic CD200 expression is present in GA, and in particular among those identified as fast progressors.
Substances chimiques
Antigens, CD
0
Biomarkers
0
antigens, CD200
UQ4V77A8VA
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
69-78Informations de copyright
© 2018 Royal Australian and New Zealand College of Ophthalmologists.