Assessment of Electrospun and Ultra-lightweight Polypropylene Meshes in the Sheep Model for Vaginal Surgery.


Journal

European urology focus
ISSN: 2405-4569
Titre abrégé: Eur Urol Focus
Pays: Netherlands
ID NLM: 101665661

Informations de publication

Date de publication:
15 01 2020
Historique:
received: 09 05 2018
revised: 21 06 2018
accepted: 16 07 2018
pubmed: 28 7 2018
medline: 21 5 2021
entrez: 28 7 2018
Statut: ppublish

Résumé

There is an urgent need to develop better materials to provide anatomical support to the pelvic floor without compromising its function. Our aim was to assess outcomes after simulated vaginal prolapse repair in a sheep model using three different materials: (1) ultra-lightweight polypropylene (PP) non-degradable textile (Restorelle) mesh, (2) electrospun biodegradable ureidopyrimidinone-polycarbonate (UPy-PC), and (3) electrospun non-degradable polyurethane (PU) mesh in comparison with simulated native tissue repair (NTR). These implants may reduce implant-related complications and avoid vaginal function loss. A controlled trial was performed involving 48 ewes that underwent NTR or mesh repair with PP, UPy-PC, or PU meshes (n=12/group). Explants were examined 60 and 180 d (six per group) post-implantation. Posterior rectovaginal dissection, NTR, or mesh repair. Implant-related complications, vaginal contractility, compliance, and host response were assessed. Power calculation and analysis of variance testing were used to enable comparison between the four groups. There were no visible implant-related complications. None of the implants compromised vaginal wall contractility, and passive biomechanical properties were similar to those after NTR. Shrinkage over the surgery area was around 35% for NTR and all mesh-augmented repairs. All materials were integrated well with similar connective tissue composition, vascularization, and innervation. The inflammatory response was mild with electrospun implants, inducing both more macrophages yet with relatively more type 2 macrophages present at an early stage than the PP mesh. Three very different materials were all well tolerated in the sheep vagina. Biomechanical findings were similar for all mesh-augmented repair and NTR. Constructs induced slightly different mid-term inflammatory profiles. Product innovation is needed to reduce implant-related complications. We tested two novel implants, electrospun and an ultra-lightweight polypropylene textile mesh, in a physiologically relevant model for vaginal surgery. All gave encouraging outcomes.

Sections du résumé

BACKGROUND
There is an urgent need to develop better materials to provide anatomical support to the pelvic floor without compromising its function.
OBJECTIVE
Our aim was to assess outcomes after simulated vaginal prolapse repair in a sheep model using three different materials: (1) ultra-lightweight polypropylene (PP) non-degradable textile (Restorelle) mesh, (2) electrospun biodegradable ureidopyrimidinone-polycarbonate (UPy-PC), and (3) electrospun non-degradable polyurethane (PU) mesh in comparison with simulated native tissue repair (NTR). These implants may reduce implant-related complications and avoid vaginal function loss.
DESIGN, SETTING, AND PARTICIPANTS
A controlled trial was performed involving 48 ewes that underwent NTR or mesh repair with PP, UPy-PC, or PU meshes (n=12/group). Explants were examined 60 and 180 d (six per group) post-implantation.
INTERVENTION
Posterior rectovaginal dissection, NTR, or mesh repair.
OUTCOME MEASUREMENTS AND STATISTICAL ANALYSIS
Implant-related complications, vaginal contractility, compliance, and host response were assessed. Power calculation and analysis of variance testing were used to enable comparison between the four groups.
RESULTS
There were no visible implant-related complications. None of the implants compromised vaginal wall contractility, and passive biomechanical properties were similar to those after NTR. Shrinkage over the surgery area was around 35% for NTR and all mesh-augmented repairs. All materials were integrated well with similar connective tissue composition, vascularization, and innervation. The inflammatory response was mild with electrospun implants, inducing both more macrophages yet with relatively more type 2 macrophages present at an early stage than the PP mesh.
CONCLUSIONS
Three very different materials were all well tolerated in the sheep vagina. Biomechanical findings were similar for all mesh-augmented repair and NTR. Constructs induced slightly different mid-term inflammatory profiles.
PATIENT SUMMARY
Product innovation is needed to reduce implant-related complications. We tested two novel implants, electrospun and an ultra-lightweight polypropylene textile mesh, in a physiologically relevant model for vaginal surgery. All gave encouraging outcomes.

Identifiants

pubmed: 30049658
pii: S2405-4569(18)30190-1
doi: 10.1016/j.euf.2018.07.024
pii:
doi:

Substances chimiques

Biocompatible Materials 0
Polypropylenes 0
Pyrimidinones 0
ureidopyrimidinone 0

Types de publication

Evaluation Study Journal Article Research Support, Non-U.S. Gov't

Langues

eng

Sous-ensembles de citation

IM

Pagination

190-198

Informations de copyright

Copyright © 2018. Published by Elsevier B.V.

Auteurs

Lucie Hympánová (L)

Centre for Surgical Technologies, Group Biomedical Sciences, KU Leuven, Leuven, Belgium; Department of Development and Regeneration, Woman and Child, Group Biomedical Sciences, KU Leuven, Leuven, Belgium; Institute for the Care of the Mother and Child, Third Faculty of Medicine, Charles University, Prague, Czech Republic.

Rita Rynkevic (R)

Centre for Surgical Technologies, Group Biomedical Sciences, KU Leuven, Leuven, Belgium; Department of Development and Regeneration, Woman and Child, Group Biomedical Sciences, KU Leuven, Leuven, Belgium; INEGI, Faculdade de Engenharia da Universidade do Porto, Universidade do Porto, Porto, Portugal.

Sabiniano Román (S)

Department of Materials and Science Engineering, Kroto Research Institute, University of Sheffield, Sheffield, UK.

Marina G M C Mori da Cunha (MGMC)

Centre for Surgical Technologies, Group Biomedical Sciences, KU Leuven, Leuven, Belgium; Department of Development and Regeneration, Woman and Child, Group Biomedical Sciences, KU Leuven, Leuven, Belgium.

Edoardo Mazza (E)

Institute of Mechanical Systems, ETH Zurich, Zurich, Switzerland; EMPA, Swiss Federal Laboratories for Materials Science and Technology, Dübendorf, Switzerland.

Manuel Zündel (M)

Institute of Mechanical Systems, ETH Zurich, Zurich, Switzerland.

Iva Urbánková (I)

Centre for Surgical Technologies, Group Biomedical Sciences, KU Leuven, Leuven, Belgium; Department of Development and Regeneration, Woman and Child, Group Biomedical Sciences, KU Leuven, Leuven, Belgium; Institute for the Care of the Mother and Child, Third Faculty of Medicine, Charles University, Prague, Czech Republic.

Monica R Gallego (MR)

Coloplast A/S, Global R&D, Biomaterials, Humlebæk, Denmark.

Jakob Vange (J)

Coloplast A/S, Global R&D, Biomaterials, Humlebæk, Denmark.

Geertje Callewaert (G)

Centre for Surgical Technologies, Group Biomedical Sciences, KU Leuven, Leuven, Belgium; Department of Development and Regeneration, Woman and Child, Group Biomedical Sciences, KU Leuven, Leuven, Belgium; Pelvic Floor Unit, University Hospitals KU Leuven, Leuven, Belgium.

Christopher Chapple (C)

The Royal Hallamshire Hospital, Sheffield, UK.

Sheila MacNeil (S)

Department of Materials and Science Engineering, Kroto Research Institute, University of Sheffield, Sheffield, UK.

Jan Deprest (J)

Centre for Surgical Technologies, Group Biomedical Sciences, KU Leuven, Leuven, Belgium; Department of Development and Regeneration, Woman and Child, Group Biomedical Sciences, KU Leuven, Leuven, Belgium; Pelvic Floor Unit, University Hospitals KU Leuven, Leuven, Belgium. Electronic address: Jan.Deprest@uzleuven.be.

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