Genetic access to neurons in the accessory optic system reveals a role for Sema6A in midbrain circuitry mediating motion perception.

Accessory optic system Medial terminal nucleus RRID: AB_2079751 RRID: IMSR_JAX:007914 RRID:AB_10000240 RRID:AB_2209751 RRID:AB_2492226 RRID:IMSR_JAX:005029 RRID:MGI:3037891 Retinal ganglion cells Sema6A.

Journal

The Journal of comparative neurology
ISSN: 1096-9861
Titre abrégé: J Comp Neurol
Pays: United States
ID NLM: 0406041

Informations de publication

Date de publication:
01 01 2019
Historique:
received: 29 05 2018
revised: 18 07 2018
accepted: 19 07 2018
pubmed: 5 8 2018
medline: 17 4 2020
entrez: 5 8 2018
Statut: ppublish

Résumé

The accessory optic system (AOS) detects retinal image slip and reports it to the oculomotor system for reflexive image stabilization. Here, we characterize two Cre lines that permit genetic access to AOS circuits responding to vertical motion. The first (Pcdh9-Cre) labels only one of the four subtypes of ON direction-selective retinal ganglion cells (ON-DS RGCs), those preferring ventral retinal motion. Their axons diverge from the optic tract just behind the chiasm and selectively innervate the medial terminal nucleus (MTN) of the AOS. Unlike most RGC subtypes examined, they survive after optic nerve crush. The second Cre-driver line (Pdzk1ip1-Cre) labels postsynaptic neurons in the MTN. These project predominantly to the other major terminal nucleus of the AOS, the nucleus of the optic tract (NOT). We find that the transmembrane protein semaphorin 6A (Sema6A) is required for the formation of axonal projections from the MTN to the NOT, just as it is for the retinal innervation of the MTN. These new tools permit manipulation of specific circuits in the AOS and show that Sema6A is required for establishing AOS connections in multiple locations.

Identifiants

pubmed: 30076594
doi: 10.1002/cne.24507
pmc: PMC6312510
mid: NIHMS1502579
doi:

Substances chimiques

Sema6a protein, mouse 0
Semaphorins 0

Types de publication

Journal Article Research Support, N.I.H., Extramural Research Support, Non-U.S. Gov't

Langues

eng

Sous-ensembles de citation

IM

Pagination

282-296

Subventions

Organisme : NINDS NIH HHS
ID : P30 NS050274
Pays : United States
Organisme : NEI NIH HHS
ID : R01 EY012793
Pays : United States
Organisme : Howard Hughes Medical Institute
Pays : United States
Organisme : NEI NIH HHS
ID : R01 EY027713
Pays : United States

Informations de copyright

© 2018 Wiley Periodicals, Inc.

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Auteurs

Brendan N Lilley (BN)

Solomon Snyder Department of Neuroscience, Howard Hughes Medical Institute, The Johns Hopkins University School of Medicine, Baltimore, Maryland.

Shai Sabbah (S)

Department of Neuroscience, Brown University, Providence, Rhode Island.

John L Hunyara (JL)

Solomon Snyder Department of Neuroscience, Howard Hughes Medical Institute, The Johns Hopkins University School of Medicine, Baltimore, Maryland.

Katherine D Gribble (KD)

Solomon Snyder Department of Neuroscience, Howard Hughes Medical Institute, The Johns Hopkins University School of Medicine, Baltimore, Maryland.

Timour Al-Khindi (T)

Solomon Snyder Department of Neuroscience, Howard Hughes Medical Institute, The Johns Hopkins University School of Medicine, Baltimore, Maryland.

Jiali Xiong (J)

Solomon Snyder Department of Neuroscience, Howard Hughes Medical Institute, The Johns Hopkins University School of Medicine, Baltimore, Maryland.

Zhuhao Wu (Z)

Laboratory of Brain Development and Repair, Rockefeller University, New York, New York.

David M Berson (DM)

Department of Neuroscience, Brown University, Providence, Rhode Island.

Alex L Kolodkin (AL)

Solomon Snyder Department of Neuroscience, Howard Hughes Medical Institute, The Johns Hopkins University School of Medicine, Baltimore, Maryland.

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