Influence of cross-linkers on ezrin-bound minimal actin cortices.
Actin network
Ezrin
Fascin
Fluorescence microscopy
Solid supported membrane
α-actinin
Journal
Progress in biophysics and molecular biology
ISSN: 1873-1732
Titre abrégé: Prog Biophys Mol Biol
Pays: England
ID NLM: 0401233
Informations de publication
Date de publication:
07 2019
07 2019
Historique:
received:
29
03
2018
revised:
13
07
2018
accepted:
31
07
2018
pubmed:
11
8
2018
medline:
20
2
2020
entrez:
11
8
2018
Statut:
ppublish
Résumé
The actin cortex is a thin network coupled to the plasma membrane of cells, responsible for e.g., cell shape, motility, growth and division. Several model systems for minimal actin cortices (MACs) have been discussed in literature trying to mimic the complex interplay of membrane and actin. We recapitulate on different types of MACs using either three dimensional droplet interfaces or lipid bilayers to which F-actin networks are attached to or planar lipid bilayers with bound actin networks. Binding of the network to the membrane interface significantly influences its properties as well as its dynamics. This in turn also influences, how cross-linkers as well as myosin motors act on the network. Here, we describe the coupling of a filamentous actin network to a model membrane via the protein ezrin, a member of the ezrin-radixin-moesin family, which forms a direct linkage between the plasma membrane and the cortical web. Ezrin binding to the membrane is achieved by the lipid PtdIns(4,5)P
Identifiants
pubmed: 30093083
pii: S0079-6107(18)30094-4
doi: 10.1016/j.pbiomolbio.2018.07.016
pii:
doi:
Substances chimiques
Actins
0
Cytoskeletal Proteins
0
Phosphatidylinositol 4,5-Diphosphate
0
ezrin
0
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
91-101Informations de copyright
Copyright © 2018 Elsevier Ltd. All rights reserved.