RNA Structure and Cellular Applications of Fluorescent Light-Up Aptamers.
RNA imaging
RNA structures
RNA-protein interactions
fluorescent light-up aptamers (FLAPs)
fluorogenic probes
Journal
Angewandte Chemie (International ed. in English)
ISSN: 1521-3773
Titre abrégé: Angew Chem Int Ed Engl
Pays: Germany
ID NLM: 0370543
Informations de publication
Date de publication:
28 01 2019
28 01 2019
Historique:
received:
05
06
2018
pubmed:
14
8
2018
medline:
5
8
2020
entrez:
14
8
2018
Statut:
ppublish
Résumé
The cellular functions of RNA are not limited to their role as blueprints for protein synthesis. In particular, noncoding RNA, such as, snRNAs, lncRNAs, miRNAs, play important roles. With increasing numbers of RNAs being identified, it is well known that the transcriptome outnumbers the proteome by far. This emphasizes the great importance of functional RNA characterization and the need to further develop tools for these investigations, many of which are still in their infancy. Fluorescent light-up aptamers (FLAPs) are RNA sequences that can bind nontoxic, cell-permeable small-molecule fluorogens and enhance their fluorescence over many orders of magnitude upon binding. FLAPs can be encoded on the DNA level using standard molecular biology tools and are subsequently transcribed into RNA by the cellular machinery, so that they can be used as fluorescent RNA tags (FLAP-tags). In this Minireview, we give a brief overview of the fluorogens that have been developed and their binding RNA aptamers, with a special focus on published crystal structures. A summary of current and future cellular FLAP applications with an emphasis on the study of RNA-RNA and RNA-protein interactions using split-FLAP and Förster resonance energy transfer (FRET) systems is given.
Identifiants
pubmed: 30102012
doi: 10.1002/anie.201806482
pmc: PMC6391945
doi:
Substances chimiques
Aptamers, Nucleotide
0
Fluorescent Dyes
0
RNA
63231-63-0
Types de publication
Journal Article
Review
Langues
eng
Sous-ensembles de citation
IM
Pagination
1266-1279Informations de copyright
© 2018 The Authors. Published by Wiley-VCH Verlag GmbH & Co. KGaA.
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