Comparison of JC-1 and MitoTracker probes for mitochondrial viability assessment in stored canine platelet concentrates: A flow cytometry study.

Animals Apoptosis / physiology Benzimidazoles / metabolism Blood Platelets / metabolism Blood Preservation / methods Carbocyanines / metabolism Dogs Flow Cytometry / methods Fluorescent Dyes / metabolism Membrane Potential, Mitochondrial / physiology Mitochondria / metabolism
JC-1 MitoTracker flow cytometry mitochondrial membrane potential platelet storage lesion

Journal

Cytometry. Part A : the journal of the International Society for Analytical Cytology
ISSN: 1552-4930
Titre abrégé: Cytometry A
Pays: United States
ID NLM: 101235694

Informations de publication

Date de publication:
02 2019
Historique:
received: 13 03 2018
revised: 18 06 2018
accepted: 27 06 2018
pubmed: 15 8 2018
medline: 9 4 2020
entrez: 15 8 2018
Statut: ppublish

Résumé

Mitochondria perform crucial roles in many biochemical processes, and mitochondrial depolarization is an early sign of platelet apoptosis. The mitochondrial membrane potential is usually evaluated through JC-1 probe, but it can also be assessed with MitoTracker probes. Our aim was to evaluate mitochondrial viability in stored canine platelet concentrates (PCs) with the fluorescent probes JC-1 and MitoTracker. Platelets from 22 canine PCs were stained with JC-1 and MitoTracker probes on days 1, 3, and 5 of storage. Data on metabolic parameters were also collected for correlation studies. Results of JC-1 and MitoTracker revealed a decrease in mitochondrial membrane potential in day 5 of storage compared to days 1 and 3, providing evidence of mitochondrial depolarization, a finding that was confirmed by the data on metabolic parameters. MitoTracker probes also added information regarding platelet swelling. In conclusion, MitoTracker probes offered a more complete mitochondrial analysis in the evaluation of stored canine PCs. © 2018 International Society for Advancement of Cytometry.

Identifiants

DOI: 10.1002/cyto.a.23567 PMID: 30107098
pubmed: 30107098
doi: 10.1002/cyto.a.23567
doi:

Substances chimiques

Benzimidazoles 0
Carbocyanines 0
Fluorescent Dyes 0
5,5',6,6'-tetrachloro-1,1',3,3'-tetraethylbenzimidazolocarbocyanine 21527-78-6

Types de publication

Comparative Study Journal Article Research Support, Non-U.S. Gov't

Langues

eng

Sous-ensembles de citation

IM

Pagination

214-218

Informations de copyright

© 2018 International Society for Advancement of Cytometry.

Auteurs

Natália Aydos Marcondes (NA)

Laboratório Zanol, Porto Alegre, Brazil.

Silvia Resende Terra (SR)

Clinical Pathology Laboratory, Veterinary Medicine Faculty, Universidade do Sul de Santa Catarina, Tubarão, Brazil.

Camila Serina Lasta (CS)

Department of Veterinary Clinical Pathology, Universidade Federal do Rio Grande do Sul, Porto Alegre, Brazil.
Department of Health, Centro Universitário Ritter dos Reis - UniRitter, Porto Alegre, Brazil.

Nicole Regina Capacchi Hlavac (NRC)

Clinical Pathology Laboratory, Veterinary Medicine Faculty, Universidade do Sul de Santa Catarina, Tubarão, Brazil.
Department of Veterinary Clinical Pathology, Universidade Federal do Rio Grande do Sul, Porto Alegre, Brazil.

Magnus Larruscaim Dalmolin (ML)

Blut's Diagnosis Center and Veterinary Services, Porto Alegre, Brazil.

Luciana de Almeida Lacerda (LA)

Blut's Diagnosis Center and Veterinary Services, Porto Alegre, Brazil.

Gustavo Adolpho Moreira Faulhaber (GAM)

Laboratório Zanol, Porto Alegre, Brazil.
Department of Internal Medicine, Universidade Federal do Rio Grande do Sul, Porto Alegre, Brazil.
Post-Graduation Program in Medicine: Medical Sciences, Universidade Federal do Rio Grande do Sul, Porto Alegre, Brazil.

Félix Hilário Díaz González (FHD)

Department of Veterinary Clinical Pathology, Universidade Federal do Rio Grande do Sul, Porto Alegre, Brazil.

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Classifications MeSH

questionsmedicales.fr › Eucaryotes › Animaux › Comparison of JC-1 and MitoTracker probes for...
questionsmedicales.fr › Phénomènes physiologiques cellulaires › Mort cellulaire › Mort cellulaire régulée › Apoptose › Comparison of JC-1 and MitoTracker probes for...
questionsmedicales.fr › Composés hétérocycliques › Composés hétérocycliques à cycles fusionnés › Composés hétérobicycliques › Benzimidazoles › Comparison of JC-1 and MitoTracker probes for...
questionsmedicales.fr › Systèmes sanguin et immunitaire › Sang › Cellules sanguines › Plaquettes › Comparison of JC-1 and MitoTracker probes for...
questionsmedicales.fr › Techniques d'investigation › Conservation biologique › Conservation de tissu › Conservation de sang › Comparison of JC-1 and MitoTracker probes for...
questionsmedicales.fr › Composés chimiques organiques › Hydrocarbures › Hydrocarbures acycliques › Alcynes › Carbocyanines › Comparison of JC-1 and MitoTracker probes for...
questionsmedicales.fr › Eucaryotes › Animaux › Chordés › Vertébrés › Mammifères › Eutheria › Carnivora › Pinnipedia › Canidae › Chiens › Comparison of JC-1 and MitoTracker probes for...
questionsmedicales.fr › Techniques d'investigation › Techniques cytologiques › Cytophotométrie › Cytométrie en flux › Comparison of JC-1 and MitoTracker probes for...
questionsmedicales.fr › Actions chimiques et utilisations › Utilisations spécialisées de produits chimiques › Produits chimiques de laboratoire › Indicateurs et réactifs › Luminescents › Colorants fluorescents › Comparison of JC-1 and MitoTracker probes for...
questionsmedicales.fr › Phénomènes physiologiques › Phénomènes électrophysiologiques › Potentiel de membrane mitochondriale › Comparison of JC-1 and MitoTracker probes for...
questionsmedicales.fr › Cellules › Structures cellulaires › Fractions subcellulaires › Mitochondries › Comparison of JC-1 and MitoTracker probes for...