Cloning, Expression, and Bioinformatics Analysis of Heavy Metal Resistance Gene afe_1862 from Acidithiobacillus ferrooxidans L1 in Escherichia coli.

Acidithiobacillus / drug effects Bacterial Proteins / genetics Computational Biology Escherichia coli / drug effects Metals, Heavy / toxicity
Acidithiobacillus ferrooxidans Bioinformatics Expression Metal resistance afe_1862

Journal

Biological trace element research
ISSN: 1559-0720
Titre abrégé: Biol Trace Elem Res
Pays: United States
ID NLM: 7911509

Informations de publication

Date de publication:
May 2019
Historique:
received: 03 06 2018
accepted: 25 07 2018
pubmed: 18 8 2018
medline: 23 7 2019
entrez: 18 8 2018
Statut: ppublish

Résumé

Molecular studies of copper and cadmium resistances in acidophilic bacteria are significant in biomining. In this study, afe_1862, which encodes a heavy metal-binding protein in Acidithiobacillus ferrooxidans L1, was amplified using PCR, cloned into the pET32a plasmid, and sequenced. Following SDS-PAGE analysis, optimization of the expression conditions and heterologous overexpression of afe_1862 in Escherichia coli BL21 in the presence of Cu

Identifiants

DOI: 10.1007/s12011-018-1462-3 PMID: 30117047
pubmed: 30117047
doi: 10.1007/s12011-018-1462-3
pii: 10.1007/s12011-018-1462-3
doi:

Substances chimiques

Bacterial Proteins 0
Metals, Heavy 0

Types de publication

Journal Article

Langues

eng

Sous-ensembles de citation

IM

Pagination

291-300

Auteurs

Feifan Leng (F)

School of Life Science and Engineering, Lanzhou University of Technology, Lanzhou, 730050, China. lff0928@sina.com.

Yuanli Li (Y)

School of Life Science and Engineering, Lanzhou University of Technology, Lanzhou, 730050, China.

Wen Luo (W)

School of Life Science and Engineering, Lanzhou University of Technology, Lanzhou, 730050, China.

Qingwei Wei (Q)

School of Life Science and Engineering, Lanzhou University of Technology, Lanzhou, 730050, China.

Yanjun Jing (Y)

School of Life Science and Engineering, Lanzhou University of Technology, Lanzhou, 730050, China.

Xiaoli Wang (X)

Lanzhou Institute of Husbandry and Pharmaceutical Science of CAAS, Lanzhou, 730050, China.

Mingjun Yang (M)

School of Life Science and Engineering, Lanzhou University of Technology, Lanzhou, 730050, China.

Yonggang Wang (Y)

School of Life Science and Engineering, Lanzhou University of Technology, Lanzhou, 730050, China. 412316788@163.com.
ORCID: 0000-0003-3762-6421

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Classifications MeSH

questionsmedicales.fr › Bactéries › Proteobacteria › Gammaproteobacteria › Acidithiobacillus › Cloning, Expression, and Bioinformatics...
questionsmedicales.fr › Acides aminés, peptides et protéines › Protéines › Protéines bactériennes › Cloning, Expression, and Bioinformatics...
questionsmedicales.fr › Sciences de l'information › Informatique › Biologie informatique › Cloning, Expression, and Bioinformatics...
questionsmedicales.fr › Bactéries › Proteobacteria › Gammaproteobacteria › Enterobacteriaceae › Escherichia › Escherichia coli › Cloning, Expression, and Bioinformatics...
questionsmedicales.fr › Produits chimiques inorganiques › Métaux › Métaux lourds › Cloning, Expression, and Bioinformatics...