Nanotechnology Enabled Modulation of Signaling Pathways Affects Physiologic Responses in Intact Vascular Tissue.
Actin Cytoskeleton
/ metabolism
Actins
/ metabolism
Animals
Blood Vessels
/ physiology
Calcium
/ metabolism
Gene Silencing
Heat-Shock Proteins
/ metabolism
Humans
Micelles
Muscle Contraction
Muscle, Smooth
/ physiology
Nanoparticles
/ chemistry
Nanotechnology
/ methods
Peptides
/ chemistry
Polymerization
RNA, Small Interfering
/ metabolism
Rats
Signal Transduction
Static Electricity
HSP20
HSP27
endosomolytic polymer
nanotechnology
smooth muscle contraction
subarachnoid hemorrhage
Journal
Tissue engineering. Part A
ISSN: 1937-335X
Titre abrégé: Tissue Eng Part A
Pays: United States
ID NLM: 101466659
Informations de publication
Date de publication:
03 2019
03 2019
Historique:
pubmed:
23
8
2018
medline:
30
8
2019
entrez:
23
8
2018
Statut:
ppublish
Résumé
Subarachnoid hemorrhage (SAH) is associated with vasospasm that is refractory to traditional vasodilators, and inhibition of vasospasm after SAH remains a large unmet clinical need. SAH causes changes in the phosphorylation state of the small heat shock proteins (HSPs), HSP20 and HSP27, in the vasospastic vessels. In this study, the levels of HSP27 and HSP20 were manipulated using nanotechnology to mimic the intracellular phenotype of SAH-induced vasospasm, and the effect of this manipulation was tested on vasomotor responses in intact tissues. This work provides insight into potential therapeutic targets for the development of more effective treatments for SAH induced vasospasm.
Identifiants
pubmed: 30132374
doi: 10.1089/ten.TEA.2018.0169
pmc: PMC6450456
doi:
Substances chimiques
Actins
0
Heat-Shock Proteins
0
Micelles
0
Peptides
0
RNA, Small Interfering
0
Calcium
SY7Q814VUP
Types de publication
Journal Article
Research Support, N.I.H., Extramural
Langues
eng
Sous-ensembles de citation
IM
Pagination
416-426Subventions
Organisme : NIBIB NIH HHS
ID : R01 EB019409
Pays : United States
Organisme : NHLBI NIH HHS
ID : R01 HL070715
Pays : United States
Organisme : NHLBI NIH HHS
ID : R01 HL122347
Pays : United States
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