Detection of Trichophyton rubrum and Trichophyton interdigitale in onychomycosis using monoclonal antibodies against Sub6 (Tri r 2).
Animals
Antibodies, Fungal
/ immunology
Antibodies, Monoclonal
/ immunology
Antigens, Fungal
/ analysis
Blotting, Western
/ methods
Enzyme-Linked Immunosorbent Assay
/ methods
Humans
Immunoassay
/ methods
Mice
Onychomycosis
/ diagnosis
Peptide Hydrolases
/ analysis
Sensitivity and Specificity
Trichophyton
/ isolation & purification
Trichophyton interdigitale
Trichophyton rubrum
Onychomycosis
Sub6
Tri r 2
monoclonal antibody
Journal
Mycoses
ISSN: 1439-0507
Titre abrégé: Mycoses
Pays: Germany
ID NLM: 8805008
Informations de publication
Date de publication:
Jan 2019
Jan 2019
Historique:
received:
13
07
2018
revised:
08
08
2018
accepted:
21
08
2018
pubmed:
29
8
2018
medline:
5
2
2019
entrez:
29
8
2018
Statut:
ppublish
Résumé
Onychomycosis is the most prevalent nail disease and is mainly caused by two dermatophyte species Trichophyton rubrum and Trichophyton interdigitale with a frequency in the range of 80% and 20%, respectively. The secreted protease Sub6 of the subtilisin family, which was never detected in vitro growth conditions, was found to be a robust marker of onychomycosis. The aim of this work was to detect tinea unguium using anti-Sub6 monoclonal antibodies in proteins extracted from clinical nail samples. We produced monoclonal antibodies in mice using recombinant Sub6 as an antigen. Selected monoclonal antibodies were tested by Western blot analysis and ELISA on protein extracts from onychomycosis samples. Several monoclonal antibodies used to quantify Sub6 in proteins extracted from clinical nail samples were produced and characterised. We showed that these antibodies were very specific and allowed the detection of T. rubrum and T. interdigitale in onychomycosis. Sub6 was detected in clinical samples infected by T. rubrum and not detected in nails with trauma and other diseases. Anti-Sub6 monoclonal antibodies could be useful for a rapid diagnosis of tinea unguium and/or therapeutic survey of dermatophyte in onychomycosis by ELISA or an immunochromatography device such as a strip test.
Sections du résumé
BACKGROUND
BACKGROUND
Onychomycosis is the most prevalent nail disease and is mainly caused by two dermatophyte species Trichophyton rubrum and Trichophyton interdigitale with a frequency in the range of 80% and 20%, respectively. The secreted protease Sub6 of the subtilisin family, which was never detected in vitro growth conditions, was found to be a robust marker of onychomycosis.
OBJECTIVE
OBJECTIVE
The aim of this work was to detect tinea unguium using anti-Sub6 monoclonal antibodies in proteins extracted from clinical nail samples.
METHODS
METHODS
We produced monoclonal antibodies in mice using recombinant Sub6 as an antigen. Selected monoclonal antibodies were tested by Western blot analysis and ELISA on protein extracts from onychomycosis samples.
RESULTS
RESULTS
Several monoclonal antibodies used to quantify Sub6 in proteins extracted from clinical nail samples were produced and characterised. We showed that these antibodies were very specific and allowed the detection of T. rubrum and T. interdigitale in onychomycosis. Sub6 was detected in clinical samples infected by T. rubrum and not detected in nails with trauma and other diseases.
CONCLUSION
CONCLUSIONS
Anti-Sub6 monoclonal antibodies could be useful for a rapid diagnosis of tinea unguium and/or therapeutic survey of dermatophyte in onychomycosis by ELISA or an immunochromatography device such as a strip test.
Substances chimiques
Antibodies, Fungal
0
Antibodies, Monoclonal
0
Antigens, Fungal
0
Peptide Hydrolases
EC 3.4.-
Banques de données
GENBANK
['LT717086', 'LAU2354']
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
32-40Subventions
Organisme : Galderma R&D
Informations de copyright
© 2018 Blackwell Verlag GmbH.