Mediator of tolerance to abiotic stress ERF6 regulates susceptibility of Arabidopsis to Meloidogyne incognita.
Adaptation, Physiological
Animals
Arabidopsis
/ genetics
Arabidopsis Proteins
/ genetics
Biosynthetic Pathways
/ genetics
Ethylenes
/ biosynthesis
Gene Expression Regulation, Plant
Genome, Plant
Plant Diseases
/ parasitology
Polymorphism, Single Nucleotide
/ genetics
Quantitative Trait Loci
/ genetics
Stress, Physiological
/ genetics
Transcription Factors
/ genetics
Tylenchoidea
/ physiology
Arabidopsis thaliana
Meloidogyne incognita
ERF6
abiotic stress
genome-wide association mapping
root-knot nematodes
transcription factor
Journal
Molecular plant pathology
ISSN: 1364-3703
Titre abrégé: Mol Plant Pathol
Pays: England
ID NLM: 100954969
Informations de publication
Date de publication:
01 2019
01 2019
Historique:
received:
24
04
2018
revised:
24
08
2018
accepted:
24
08
2018
pubmed:
31
8
2018
medline:
9
8
2019
entrez:
31
8
2018
Statut:
ppublish
Résumé
Root-knot nematodes transform vascular host cells into permanent feeding structures to selectively withdraw their nutrients from host plants during the course of several weeks. The susceptibility of host plants to root-knot nematode infections is thought to be a complex trait involving many genetic loci. However, genome-wide association (GWA) analysis has so far revealed only four quantitative trait loci (QTLs) linked to the reproductive success of the root-knot nematode Meloidogyne incognita in Arabidopsis thaliana, which suggests that the genetic architecture underlying host susceptibility could be much simpler than previously thought. Here, we report that, by using a relaxed stringency approach in a GWA analysis, we could identify 15 additional loci linked to quantitative variation in the reproductive success of M. incognita in Arabidopsis. To test the robustness of our analysis, we functionally characterized six genes located in a QTL with the lowest acceptable statistical support and smallest effect size. This led us to identify ETHYLENE RESPONSE FACTOR 6 (ERF6) as a novel susceptibility gene for M. incognita in Arabidopsis. ERF6 functions as a transcriptional activator and suppressor of genes in response to various abiotic stresses independent of ethylene signalling. However, whole-transcriptome analysis of nematode-infected roots of the Arabidopsis erf6-1 knockout mutant line showed that allelic variation at this locus may regulate the conversion of aminocyclopropane-1-carboxylate (ACC) into ethylene by altering the expression of 1-aminocyclopropane-1-carboxylate oxidase 3 (ACO3). Our data further suggest that tolerance to abiotic stress mediated by ERF6 forms a novel layer of control in the susceptibility of Arabidopsis to M. incognita.
Identifiants
pubmed: 30160354
doi: 10.1111/mpp.12745
pmc: PMC6430479
doi:
Substances chimiques
Arabidopsis Proteins
0
ERF6 protein, Arabidopsis
0
Ethylenes
0
Transcription Factors
0
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Pagination
137-152Informations de copyright
© 2018 The Authors. Molecular Plant Pathology published by BSPP and John Wiley & Sons Ltd.
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