Antigen host response differences between the animal-type strain and human-clinical Pythium insidiosum isolates used for serological diagnosis in Thailand.
Pythium insidiosum
Antigenic differences
Serological diagnosis
Journal
Medical mycology
ISSN: 1460-2709
Titre abrégé: Med Mycol
Pays: England
ID NLM: 9815835
Informations de publication
Date de publication:
01 Jun 2019
01 Jun 2019
Historique:
received:
14
03
2018
revised:
20
07
2018
accepted:
28
07
2018
pubmed:
31
8
2018
medline:
30
4
2019
entrez:
31
8
2018
Statut:
ppublish
Résumé
The detection of Pythium insidiosum-specific-immunoglobulin-G antibody (Pi-Ab) with enzyme-linked immunosorbent assay (ELISA) test depends on the source of antigen. In this study, the Pi-Ab levels in 140 serum samples from patients with pythiosis were evaluated by ELISA using antigens from 10 P. insidiosum clinical isolates in comparison with antigen from the equine-standard-type strain. The ELISA values (EVs), calculated from antibody levels from serum of patients with pythiosis or other infections versus healthy controls, were significantly higher in the test with clinical-isolates antigen than the standard-equine-type strain (6.0 ± 2.6 vs 4.0 ± 1.7, respectively; P < .0001). ELISA with antigen from human source might be more proper diagnosis test.
Identifiants
pubmed: 30165659
pii: 5079160
doi: 10.1093/mmy/myy072
doi:
Substances chimiques
Antibodies, Fungal
0
Antigens, Fungal
0
Immunoglobulin G
0
Types de publication
Evaluation Study
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
519-522Informations de copyright
© The Author(s) 2018. Published by Oxford University Press on behalf of The International Society for Human and Animal Mycology.