Post-mortem in situ stability of serum markers of cerebral damage and acute phase response.
Acute-Phase Reaction
Adult
Aged
Aged, 80 and over
Biomarkers
/ blood
Brain Injuries
/ blood
Brain-Derived Neurotrophic Factor
/ blood
C-Reactive Protein
/ analysis
Female
Ferritins
/ blood
Glial Fibrillary Acidic Protein
/ blood
Humans
Immunoassay
Interleukin-6
/ blood
L-Lactate Dehydrogenase
/ blood
Male
Middle Aged
Phosphopyruvate Hydratase
/ blood
Postmortem Changes
Procalcitonin
/ blood
Prospective Studies
Receptors, Tumor Necrosis Factor, Type I
/ blood
S100 Calcium Binding Protein beta Subunit
/ blood
Acute phase response
Intra-individual stability
Post-mortem biochemistry
Serum
Thanatochemistry
Traumatic brain injury
Journal
International journal of legal medicine
ISSN: 1437-1596
Titre abrégé: Int J Legal Med
Pays: Germany
ID NLM: 9101456
Informations de publication
Date de publication:
May 2019
May 2019
Historique:
received:
05
07
2018
accepted:
23
08
2018
pubmed:
1
9
2018
medline:
30
5
2019
entrez:
1
9
2018
Statut:
ppublish
Résumé
The aim of the given study was to test the in situ stability of biochemical markers of cerebral damage and acute phase response in the early post-mortem interval to assess their usability for forensic pathology. A monocentric, prospective study investigated post-mortem femoral venous blood samples at four time points obtained within 48 h post-mortem starting at the death of 20 deceased, using commercial immunoassays for the ten parameters: S100 calcium-binding protein B (S100B), glial fibrillary acidic protein (GFAP), neuron-specific enolase (NSE), brain-derived neurotrophic factor (BDNF), interleukin-6 (IL-6), C-reactive protein (CRP), procalcitonin (PCT), ferritin, soluble tumor necrosis factor receptor type 1 (sTNFR1), and lactate dehydrogenase (LDH). Significant changes in serum levels were observed only later than 2 h after death for all markers. Inter-laboratory comparability was high, and intra-assay precision was sufficient for most markers. Most of the biomarker levels depended on the severity of hemolysis and lipemia but were robust against freeze-thaw cycles. Serum levels increased with longer post-mortem intervals for S100B, NSE, ferritin, sTNFR1, and LDH (for all p < 0.001) but decreased over this period for CRP (p = 0.089) and PCT (p < 0.001). Largely unchanged median values were found for GFAP (p = 0.139), BDNF (p = 0.106), and IL-6 (p = 0.094). Serum levels of CRP (p = 0.059) and LDH (p = 0.109) did not differ significantly between the final ante-mortem (resuscitation) and the first post-mortem sample (moment of death). Collecting the post-mortem blood sample as soon as possible will reduce the influence of post-mortem blood changes. Serum GFAP for detection of cerebral damage as well as serum IL-6 and CRP as proof of acute phase response seemed to be preferable due to their in situ stability in the first 2 days after death.
Identifiants
pubmed: 30167776
doi: 10.1007/s00414-018-1925-2
pii: 10.1007/s00414-018-1925-2
doi:
Substances chimiques
Biomarkers
0
Brain-Derived Neurotrophic Factor
0
Glial Fibrillary Acidic Protein
0
Interleukin-6
0
Procalcitonin
0
Receptors, Tumor Necrosis Factor, Type I
0
S100 Calcium Binding Protein beta Subunit
0
S100B protein, human
0
C-Reactive Protein
9007-41-4
Ferritins
9007-73-2
L-Lactate Dehydrogenase
EC 1.1.1.27
Phosphopyruvate Hydratase
EC 4.2.1.11
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
871-881Références
Coe J (1993) Post-mortem chemistry update. Emphasis on forensic application. Am J Forensic Med Pathol 14:91–111
pubmed: 8328447
Palmiere C, Mangin P (2012a) Post-mortem chemistry update part I. Int J Legal Med 126:187–198
pubmed: 21947676
Palmiere C, Mangin P (2012b) Post-mortem chemistry update part II. Int J Legal Med 126:199–215
pubmed: 21984165
Maeda H, Zhu BL, Ishikawa T, Quan L, Michiue T (2009) Significance of post-mortem biochemistry in determining the cause of death. Legal Med 11:46–49
Luna A (2009) Is post-mortem biochemistry really useful? Why is it not widely used in forensic pathology? Legal Med 11:27–30
Ondruschka B, Schuch S, Pohlers D, Franke H, Dreßler J (2018) Acute phase response after fatal traumatic brain injury. Int J Legal Med 132:531–539.
pubmed: 29306988
Ondruschka B, Sieber M, Kirsten H; Franke H, Dreßler J (2018) Measurement of cerebral biomarkers proving traumatic brain injuries in post-mortem body fluids. J Neurotrauma 35:2044–2055.
pubmed: 29732941
Sieber M, Dreßler J, Franke, Pohlers D, Ondruschka B (2018) Post-mortem biochemistry of NSE and S100B. A supplemental tool for detecting a lethal traumatic brain injury? J Forensic Legal Med 55:65–73.
Woodcock T, Morganti-Kossmann MC (2013) The role of markers of inflammation in traumatic brain injury. Front Neurol 4:18
pubmed: 23459929
pmcid: 3586682
Tsokos M, Reichelt U, Jung R, Nierhaus A, Püschel K (2001) Interleukin-6 and C-reactive protein serum levels in sepsis-related fatalities during the early postmortem period. Forensic Sci Int 119:47–56.
pubmed: 11348793
Tsokos M, Reichelt U, Nierhaus A, Püschel K (2001) Serum procalcitonin (PCT): a valuable biochemical parameter for the post-mortem diagnosis of sepsis. Int J Legal Med 114:237–243.
pubmed: 11355402
Soar J, Nolan JP, Böttiger BW, Perkins GD, Loot C, Carli P, Pellis T, Sandroni C, Skrifvars MB, Smith GB, Sunde K, Deakin CD et al (2015) European resuscitation council guidelines for resuscitation 2015. Section 3. Adult advanced life support. Resuscitation 95:100–147
pubmed: 26477701
Hess C, Krueger L, Unger M, Madea B (2017) Freeze- and -thaw stability and long-term-stability of 84 synthetic cannabinoids in serum. Drug Test Anal 9:1506–1511
pubmed: 27863089
German Medical Assocation (2015) Revision of the “Guideline of the German Medical Association on quality assurance in medical laboratory examinations - Rili-BAEK”. J Lab Med 39:26–69
Ishikawa T, Hamel M, Zhu B-L, Li D-R, Zhao D, Michiue T, Maeda H (2008) Comparative evaluation of postmortem serum concentrations of neopterin and C-reactive protein. Forensic Sci Int 179:135–143
pubmed: 18541395
Palmiere C, Bardy D, Mangin P, Augsburger M (2013) Value of sTREM-1, procalcitonin and CRP as laboratory parameters for postmortem diagnosis of sepsis. J Inf Secur 67:545–555
Bode-Jänisch S, Schütz S, Schmidt A, Tschernig T, Debertin AS, Fieguth A, Hagemeier L, Teske J, Suerbaum S, Klintschar M, Bange FC (2013) Serum procalcitonin levels in the postmortem diagnosis of sepsis. Forensic Sci Int 226:266–272
pubmed: 23434379
Majdan M, Plancikova D, Brazinova A, Rusnak M, Nieboer D, Feigin V, Maas A (2016) Epidemiology of traumatic brain injuries in Europe: a cross-sectional analysis. Lancet Public Health 1:e76–e83
pubmed: 29253420
Blennow K, Brody DL, Kochanek PM, Levin H, McKee A, Ribbers GM, Yaffe K, Zetterberg H (2016) Traumatic brain injuries. Nat Rev Dis Primers 2:16084
pubmed: 27853132
Chirica VI (2017) Useful markers to assess traumatic and hypoxic brain injury. Rom J Legal Med 25:146–151
Wang KK, Yang Z, Zhu T, Shi Y, Rubenstein R, Tyndall JA, Manley GT (2018) An update on diagnostic and prognostic biomarkers for traumatic brain injury. Expert Rev Mol Diagn 18:165–180
pubmed: 29338452
pmcid: 6359936
Li DR, Zhu BL, Ishikawa T, Zhao D, Michiue T, Maeda H (2006a) Postmortem serum protein S100B levels with regard to the cause of death involving brain damage in medicolegal autopsy cases. Legal Med 8:71–77
pubmed: 16337822
Li DR, Zhu BL, Ishikawa T, Zhao D, Michiue T, Maeda H (2006b) Immunohistochemical distribution of S-100 protein in the cerebral cortex with regard to the cause of death in forensic autopsy. Legal Med 8:78–85
pubmed: 16338157
Goede A, Dreßler J, Sommer G, Schober K, Franke H, Ondruschka B (2015) Wundalterdiagnostik nach letalem Schädel-Hirn-Trauma. Rechtsmedizin 25:261–267
Ondruschka B, Pohlers D, Sommer G, Schober K, Teupser D, Franke H, Dreßler J (2013) S100B and NSE as useful postmortem biochemical markers of traumatic brain injury in autopsy cases. J Neurotrauma 30:1862–1871
Osuna E, Perez-Carceles MD, Luna A, Pounder DJ (1992) Efficacy of cerebro-spinal fluid biochemistry in the diagnosis of brain insult. Forensic Sci Int 52:193–198
pubmed: 1601350
Raabe A, Kopetsch O, Groß U, Zimmermann M, Gebhart P (2003) Measurements of serum S-100B protein: effects of storage time and temperature on pre-analytical stability. Clin Chem Lab Med 41:700–703
pubmed: 12812270
Beaudeux JL, Léger P, Dequen L, Gandjbakhch I, Coriat P, Foglietti MJ (2000) Influence of hemolysis on the measurement of S-100 protein and neuron-specific enolase plasma concentrations during coronary artery bypass grafting. Clin Chem 46:989–990
pubmed: 10894842
Missler U, Wiesmann M, Wittmann G (1999) Measurement of glial fibrillary acidic protein in human blood: analytical method and preliminary clinical results. Clin Chem 45:138–141
pubmed: 9895354
Ramont L, Thoannes H, Volondat, Chastang F, Millet MC, Maquart FX (2005) Effects of hemolysis and storage condition on neuron-specific enolase (NSE) in cerebrospinal fluid and serum: implications in clinical practice. Clin Chem Lab Med 43:1215–1217
pubmed: 16232088
Verfaillie CJ, Delanghe JR (2010) Hemolysis correction factor in the measurement of serum neuron-specific enolase. Clin Chem Lab Med 48:891–892
pubmed: 20345234
Korley FK, Diaz-Arrastia R, Wu AH, Yue JK, Manley GT, Sair HI, Van Eyk J, Everett AD et al (2016) Circulating brain-derived neurotrophic factor has diagnostic and prognostic value in traumatic brain injury. J Neurotrauma 33:215–225
pubmed: 26159676
pmcid: 4722554
Failla MD, Conley YP, Wagner AK (2016) Brain-derived neurotrophic factor (BDNF) in traumatic brain injury-related mortality: interrelationships between genetics and acute systemic and central nervous system BDNF profiles. Neurorehabil Neural Repair 30:83–93
pubmed: 25979196
Tsokos M (2007) Postmortem diagnosis of sepsis. Forensic Sci Int 165:155–164
pubmed: 16787725
Mimasaka S, Funayama M, Hashiyada M, Nata M, Tsunenari S (2007) Significance of levels of IL-6 and IL-8 after trauma. A study of 11 cytokines post-mortem using multiplex immunoassay. Injury 38:1047–1051
pubmed: 17574251
Schrag B, Roux-Lombard P, Schneiter D, Vaucher P, Mangin P, Palmiere C (2012) Evaluation of C-reactive protein, procalcitonin, tumor necrosis factor alpha, interleukin-6, and interleukin-8 as diagnostic parameters in sepsis-related fatalities. Int J Legal Med 126:505–512
pubmed: 21735293
Fujita MQ, Zhu B-L, Ishida K, Quan L, Oritani S, Maeda H (2002) Serum C-reactive protein levels in postmortem blood—an analysis with special reference to the cause of death and survival time. Forensic Sci Int 130:160–166
pubmed: 12477638
Astrup BS, Thomsen JL (2007) The routine use of C-reactive protein in forensic investigations. Forensic Sci Int 172:49–55
pubmed: 17222998
Maeda H, Zhu B-L, Bessho Y, Ishikawa T, Quan L, Michiue T, Zhao D, Li D-R, Komatsu A (2008) Postmortem serum nitrogen compounds and C-reactive protein levels with special regard to investigation of fatal hyperthermia. Forensic Sci Med Pathol 4:175–180
pubmed: 19291458
Uhlin-Hansen L (2001) C-reactive protein (CRP), a comparison of pre- and post-mortem blood levels. Forensic Sci Int 124:32–35
pubmed: 11741757
Palmiere C, Augsburger M (2014) Markers for sepsis diagnosis in the forensic setting. State of the art. Croat Med J 55:103–114
pubmed: 24778096
Schmidt S, Windgassen M, Nofer J-R, Pfeiffer H, Ribbecke S, Schmeling A (2015) Procalcitonin as a postmortem sepsis marker. A comparison of the validity of results obtained from blood serum, aqueous humour and cerebrospinal fluid. Int J Legal Med 129:117–123
pubmed: 25119683
Ramsthaler F, Kettner M, Mall G, Bratzke H (2008) The use of rapid diagnostic test of procalcitonin serum levels for the postmortem diagnosis of sepsis. Forensic Sci Int 178:139–145
pubmed: 18450398
Meisner M, Tschaikowsky K, Schnabel S, Schmidt J, Katalinic A, Schüttler J (1997) Procalcitonin - influence of temperature, storage, anticoagulation and arterial or venous asservation of blood samples on procalcitonin concentrations. Clin Chem Lab Med 35:597–602.
Madea B, Musshoff F (2007) Post-mortem biochemistry. Forensic Sci Int 165:165–171
pubmed: 16781101
Flanagan RJ (2017) Post-mortem biochemistry and toxicology. Arab J For Sci For Med 1:540–552
Hanrieder J, Wetterhall M, Enblad P, Hillered L, Bergquist J (2009) Temporally resolved differential proteomic analysis of human ventricular CSF for monitoring traumatic brain injury biomarker candidates. J Neurosci Methods 177:469–478
pubmed: 19263575