Paracrine potential of adipose stromal vascular fraction cells to recover hypoxia-induced loss of cardiomyocyte function.


Journal

Biotechnology and bioengineering
ISSN: 1097-0290
Titre abrégé: Biotechnol Bioeng
Pays: United States
ID NLM: 7502021

Informations de publication

Date de publication:
01 2019
Historique:
received: 24 05 2018
revised: 02 08 2018
accepted: 30 08 2018
pubmed: 2 9 2018
medline: 18 12 2019
entrez: 2 9 2018
Statut: ppublish

Résumé

Cell-based therapies show promising results in cardiac function recovery mostly through paracrine-mediated processes (as angiogenesis) in chronic ischemia. In this study, we aim to develop a 2D (two-dimensional) in vitro cardiac hypoxia model mimicking severe cardiac ischemia to specifically investigate the prosurvival paracrine effects of adipose tissue-derived stromal vascular fraction (SVF) cell secretome released upon three-dimensional (3D) culture. For the 2D-cardiac hypoxia model, neonatal rat cardiomyocytes (CM) were cultured for 5 days at < 1% (approaching anoxia) oxygen (O

Identifiants

pubmed: 30171703
doi: 10.1002/bit.26824
doi:

Types de publication

Journal Article Research Support, Non-U.S. Gov't

Langues

eng

Sous-ensembles de citation

IM

Pagination

132-142

Informations de copyright

© 2018 Wiley Periodicals, Inc.

Auteurs

Myroslava Mytsyk (M)

Department of Surgery, University Hospital Basel, Basel, Switzerland.
Department of Biomedicine, University of Basel, Basel, Switzerland.

Giuseppe Isu (G)

Department of Surgery, University Hospital Basel, Basel, Switzerland.
Department of Biomedicine, University of Basel, Basel, Switzerland.

Giulia Cerino (G)

Department of Surgery, University Hospital Basel, Basel, Switzerland.
Department of Biomedicine, University of Basel, Basel, Switzerland.

Martin T R Grapow (MTR)

Department of Surgery, University Hospital Basel, Basel, Switzerland.

Friedrich S Eckstein (FS)

Department of Surgery, University Hospital Basel, Basel, Switzerland.

Anna Marsano (A)

Department of Surgery, University Hospital Basel, Basel, Switzerland.
Department of Biomedicine, University of Basel, Basel, Switzerland.

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Classifications MeSH