Fsk and IBMX inhibit proliferation and proapoptotic of glioma stem cells via activation of cAMP signaling pathway.
1-Methyl-3-isobutylxanthine
/ pharmacology
Apoptosis
/ drug effects
Brain Neoplasms
/ metabolism
Butadienes
/ pharmacology
Cell Line, Tumor
Cell Proliferation
/ drug effects
Colforsin
/ pharmacology
Cyclic AMP
/ metabolism
Cyclic AMP Response Element-Binding Protein
/ metabolism
Glioma
/ metabolism
Humans
Mitogen-Activated Protein Kinases
/ metabolism
Neoplastic Stem Cells
/ metabolism
Nitriles
/ pharmacology
Plant Extracts
/ pharmacology
Plant Roots
/ chemistry
Plectranthus
/ chemistry
Signal Transduction
/ drug effects
Fsk-IBMX
GSCs
MAPK
cAMP
proliferation
signaling pathway
Journal
Journal of cellular biochemistry
ISSN: 1097-4644
Titre abrégé: J Cell Biochem
Pays: United States
ID NLM: 8205768
Informations de publication
Date de publication:
01 2019
01 2019
Historique:
received:
14
11
2017
accepted:
26
06
2018
pubmed:
2
9
2018
medline:
26
2
2020
entrez:
2
9
2018
Statut:
ppublish
Résumé
We aimed to find out the underlying mechanism of forskolin (Fsk) and 3-isobutyl-1-methylxanthine (IBMX) on glioma stem cells (GSCs). The expression of cAMP-related protein CREB and pCREB as well as apoptosis-related proteins were detected through Western blot analysis. The level of proliferation and growth rate of human GSCs was measured through thiazolyl blue tetrazolium bromide assay and stem cells forming sphere assay. The apoptosis-related gene expression was measured through reverse transcription-polymerase chain reaction. cAMP signaling pathway was activated in GSCs with Fsk-IBMX administration. Fsk-IBMX could inhibit the proliferation as well as invasion and promote the apoptosis of U87 cells. Besides, U0126 could inhibit MAPK signaling pathway to increase the sensitivity of GSCs to cAMP signaling pathway. As a result, Fsk-IBMX combined with U0126 had more negative effect on GSCs. The relationship of cAMP and MAPK signaling pathway in GSCs may provide a potential therapeutic strategy in glioma.
Substances chimiques
Butadienes
0
CREB1 protein, human
0
Cyclic AMP Response Element-Binding Protein
0
Nitriles
0
Plant Extracts
0
U 0126
0
Colforsin
1F7A44V6OU
Cyclic AMP
E0399OZS9N
Mitogen-Activated Protein Kinases
EC 2.7.11.24
1-Methyl-3-isobutylxanthine
TBT296U68M
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
321-331Informations de copyright
© 2018 Wiley Periodicals, Inc.