In Vivo Femtosecond Laser Subsurface Cortical Microtransections Attenuate Acute Rat Focal Seizures.
4-Aminopyridine
Animals
Cerebral Cortex
Disease Models, Animal
Electrophysiological Phenomena
Fluorescamine
Indicators and Reagents
Laser Therapy
/ methods
Microsurgery
/ methods
Neurosurgical Procedures
Optical Imaging
Potassium Channel Blockers
Rats
Seizures
/ physiopathology
Somatosensory Cortex
/ physiopathology
Tail
Touch Perception
2-photon imaging
4-aminopyridine
epilepsy model
laser
microsurgery
Journal
Cerebral cortex (New York, N.Y. : 1991)
ISSN: 1460-2199
Titre abrégé: Cereb Cortex
Pays: United States
ID NLM: 9110718
Informations de publication
Date de publication:
22 07 2019
22 07 2019
Historique:
received:
13
06
2018
revised:
03
08
2018
pubmed:
8
9
2018
medline:
16
7
2020
entrez:
8
9
2018
Statut:
ppublish
Résumé
Recent evidence shows that seizures propagate primarily through supragranular cortical layers. To selectively modify these circuits, we developed a new technique using tightly focused, femtosecond infrared laser pulses to make as small as ~100 µm-wide subsurface cortical incisions surrounding an epileptic focus. We use this "laser scalpel" to produce subsurface cortical incisions selectively to supragranular layers surrounding an epileptic focus in an acute rodent seizure model. Compared with sham animals, these microtransections completely blocked seizure initiation and propagation in 1/3 of all animals. In the remaining animals, seizure frequency was reduced by 2/3 and seizure propagation reduced by 1/3. In those seizures that still propagated, it was delayed and reduced in amplitude. When the recording electrode was inside the partially isolated cube and the seizure focus was on the outside, the results were even more striking. In spite of these microtransections, somatosensory responses to tail stimulation were maintained but with reduced amplitude. Our data show that just a single enclosing wall of laser cuts limited to supragranular layers led to a significant reduction in seizure initiation and propagation with preserved cortical function. Modification of this concept may be a useful treatment for human epilepsy.
Identifiants
pubmed: 30192931
pii: 5091391
doi: 10.1093/cercor/bhy210
pmc: PMC6644864
doi:
Substances chimiques
Indicators and Reagents
0
Potassium Channel Blockers
0
Fluorescamine
38183-12-9
4-Aminopyridine
BH3B64OKL9
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
3415-3426Subventions
Organisme : NINDS NIH HHS
ID : R01 NS108472
Pays : United States
Organisme : NINDS NIH HHS
ID : R21 NS078644
Pays : United States
Organisme : NCRR NIH HHS
ID : UL1 RR024996
Pays : United States
Informations de copyright
© The Author(s) 2018. Published by Oxford University Press. All rights reserved. For Permissions, please e-mail: journals.permissions@oup.com.
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