Interferon tau-dependent and independent effects of the bovine conceptus on the endometrial transcriptome†.
Animals
Cattle
/ embryology
Endometrium
/ metabolism
Estrus Synchronization
Female
Gene Expression Regulation
Gonadotropin-Releasing Hormone
Insemination, Artificial
Interferon Type I
/ metabolism
Pregnancy
Pregnancy Proteins
/ metabolism
Pregnancy, Animal
Progesterone
/ administration & dosage
Superovulation
Transcriptome
RNAseq
conceptus
endometrium
explant
interferon tau
transcriptome
Journal
Biology of reproduction
ISSN: 1529-7268
Titre abrégé: Biol Reprod
Pays: United States
ID NLM: 0207224
Informations de publication
Date de publication:
01 02 2019
01 02 2019
Historique:
received:
28
06
2018
revised:
14
08
2018
accepted:
07
09
2018
pubmed:
12
9
2018
medline:
31
3
2020
entrez:
12
9
2018
Statut:
ppublish
Résumé
This study investigated bovine conceptus-induced modifications to the endometrial transcriptome related to effects of interferon tau (IFNT), conceptus origin (in vivo vs. in vitro), and conceptus sex. In vitro (IVF) or in vivo (superovulation and artificial insemination, AI) produced blastocysts were transferred into recipient heifers on day 7 of the estrous cycle. On day 15, IVF- or AI-derived conceptuses were obtained by uterine flushing and individually placed on endometrial explants in media for 6 h. Explants were also cultured with media alone as a control or media containing 100 ng/mL IFNT. Total explant RNA was analyzed by RNA-Seq. Incubation of endometrium with IFNT or IVF- or AI-derived conceptuses changed (P ≤ 0.001) expression of 491, 498, and 576 transcripts, respectively, compared to the control. Further, 369 differentially expressed genes (DEGs) were common between explants exposed to IFNT or a conceptus. A total of 240 DEGs were uniquely altered by conceptuses (IVF- and AI-derived) but not IFNT. Of these transcripts, 46 were shared between the IVF and AI groups, while 61 and 133 were specific to IVF and AI conceptuses, respectively. Five genes [melanophilin (MLPH), prominin-2 (PROM2), myeloid associated differentiation marker (MYADM), vomeronasal 1 receptor 4 like (VN1R4L) and 5-hydroxytryptamine receptor 1A (HTR1A)] were more abundant in endometrium exposed to female compared to male conceptuses (P < 0.001). A single gene [ADP-ribosylation factor like GTPase 4C (ARL4C)] was more abundant in response to male conceptuses (P < 0.001) than female conceptuses. These data support the hypothesis that conceptus regulation of gene expression in the endometrium is complex and involves factors other than IFNT that may have a biological role in pregnancy establishment.
Identifiants
pubmed: 30203055
pii: 5094552
doi: 10.1093/biolre/ioy199
pmc: PMC6378860
doi:
Substances chimiques
Interferon Type I
0
Pregnancy Proteins
0
interferon tau
0
Gonadotropin-Releasing Hormone
33515-09-2
Progesterone
4G7DS2Q64Y
Types de publication
Journal Article
Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
365-380Subventions
Organisme : NICHD NIH HHS
ID : R01 HD072898
Pays : United States
Informations de copyright
© The Author(s) 2018. Published by Oxford University Press on behalf of Society for the Study of Reproduction.
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