A novel cell transfection platform based on laser optoporation mediated by Au nanostar layers.
Au nanoparticles
Au nanostar layers
HeLa cells
cell transfection
optoporation
plasmid DNA
Journal
Journal of biophotonics
ISSN: 1864-0648
Titre abrégé: J Biophotonics
Pays: Germany
ID NLM: 101318567
Informations de publication
Date de publication:
01 2019
01 2019
Historique:
received:
07
05
2018
revised:
26
07
2018
accepted:
09
09
2018
pubmed:
12
9
2018
medline:
26
3
2020
entrez:
12
9
2018
Statut:
ppublish
Résumé
The recently developed laser-induced cell transfection mediated by Au nanoparticles is a promising alternative to the well-established lipid-based transfection or to electroporation. Optoporation is based on the laser plasmonic heating of nanoparticles located near the cell membrane. However, the uncontrollable cell damage from intense laser pulses and from random attachment of nanoparticles may be crucial for transfection. We present a novel plasmonic optoporation technique that uses Au nanostar layers immobilized in culture microplate wells. HeLa cells were grown directly on Au nanostar layers, after which they were subjected to continuous-wave 808 nm laser irradiation. An Au monolayer density ~15 μg/cm
Identifiants
pubmed: 30203552
doi: 10.1002/jbio.201800166
doi:
Substances chimiques
Coloring Agents
0
Dextrans
0
Gold
7440-57-5
DNA
9007-49-2
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
e201800166Informations de copyright
© 2018 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.