cDNA cloning, heterologous expression, protein folding and immunogenic properties of a phospholipase A

Animals Bothrops / genetics Cloning, Molecular Crotalid Venoms / biosynthesis DNA, Complementary Escherichia coli / enzymology Gene Expression Horses / immunology Phospholipases A2 / biosynthesis Protein Folding
Antibodies Bothrops ammodytoides Phospholipase Protein expression Snake Venom Viper

Journal

Protein expression and purification
ISSN: 1096-0279
Titre abrégé: Protein Expr Purif
Pays: United States
ID NLM: 9101496

Informations de publication

Date de publication:
02 2019
Historique:
received: 06 04 2018
revised: 07 09 2018
accepted: 07 09 2018
pubmed: 12 9 2018
medline: 24 1 2020
entrez: 12 9 2018
Statut: ppublish

Résumé

A mRNA transcript that codes for a phospholipase (PLA

Identifiants

DOI: 10.1016/j.pep.2018.09.004 PMID: 30205154
pubmed: 30205154
pii: S1046-5928(18)30166-9
doi: 10.1016/j.pep.2018.09.004
pii:
doi:

Substances chimiques

Crotalid Venoms 0
DNA, Complementary 0
Phospholipases A2 EC 3.1.1.4

Types de publication

Journal Article Research Support, Non-U.S. Gov't

Langues

eng

Sous-ensembles de citation

IM

Pagination

33-43

Informations de copyright

Copyright © 2018 Elsevier Inc. All rights reserved.

Auteurs

Herlinda Clement (H)

Departamento de Medicina Molecular y Bioprocesos, Instituto de Biotecnologia, Universidad Nacional Autónoma de México, Avenida Universidad 2001, Apartado Postal 510-3, Cuernavaca Mor, 62210, Mexico; Centro de Investigación en Biotecnología, Universidad Autónoma del Estado de Morelos, Av. Universidad 2001, Cuernavaca Mor, 62209, Mexico.

Gerardo Corzo (G)

Departamento de Medicina Molecular y Bioprocesos, Instituto de Biotecnologia, Universidad Nacional Autónoma de México, Avenida Universidad 2001, Apartado Postal 510-3, Cuernavaca Mor, 62210, Mexico.

Edgar Neri-Castro (E)

Departamento de Medicina Molecular y Bioprocesos, Instituto de Biotecnologia, Universidad Nacional Autónoma de México, Avenida Universidad 2001, Apartado Postal 510-3, Cuernavaca Mor, 62210, Mexico.

Ivan Arenas (I)

Departamento de Medicina Molecular y Bioprocesos, Instituto de Biotecnologia, Universidad Nacional Autónoma de México, Avenida Universidad 2001, Apartado Postal 510-3, Cuernavaca Mor, 62210, Mexico.

Silvia Hajos (S)

Cátedra de Inmunología, Facultad de Farmacia y Bioquímica- IDEHU. UBA-CONICET, Buenos Aires, Argentina.

Adolfo R de Roodt (AR)

Laboratorio de Toxinopatología, Centro de Patología Experimental y Aplicada, Facultad de Medicina, Universidad de Buenos Aires, Argentina; Instituto Nacional de Producción de Biológicos, A.N.L.I.S. "Dr. Carlos G. Malbrán", Ministerio de Salud, Buenos Aires, Argentina.

Elba Villegas (E)

Centro de Investigación en Biotecnología, Universidad Autónoma del Estado de Morelos, Av. Universidad 2001, Cuernavaca Mor, 62209, Mexico. Electronic address: elbav@uaem.mx.

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Classifications MeSH

questionsmedicales.fr › Eucaryotes › Animaux › cDNA cloning, heterologous expression, protein...
questionsmedicales.fr › Eucaryotes › Animaux › Chordés › Vertébrés › Reptiles › Serpents › Alethinophidia › Viperidae › Crotalinae › Bothrops › cDNA cloning, heterologous expression, protein...
questionsmedicales.fr › Techniques d'investigation › Techniques génétiques › Clonage moléculaire › cDNA cloning, heterologous expression, protein...
questionsmedicales.fr › Facteurs biologiques › Toxines biologiques › Venins › Venins de serpent › Venins de vipère › Venins de crotalidé › cDNA cloning, heterologous expression, protein...
questionsmedicales.fr › Actions chimiques et utilisations › Utilisations spécialisées de produits chimiques › Produits chimiques de laboratoire › Sondes moléculaires › Sondes d'acide nucléique › Sondes d'ADN › ADN complémentaire › cDNA cloning, heterologous expression, protein...
questionsmedicales.fr › Bactéries › Proteobacteria › Gammaproteobacteria › Enterobacteriaceae › Escherichia › Escherichia coli › cDNA cloning, heterologous expression, protein...
questionsmedicales.fr › Phénomènes génétiques › Expression des gènes › cDNA cloning, heterologous expression, protein...
questionsmedicales.fr › Eucaryotes › Animaux › Chordés › Vertébrés › Mammifères › Eutheria › Perissodactyla › Equidae › Equus caballus › cDNA cloning, heterologous expression, protein...
questionsmedicales.fr › Enzymes et coenzymes › Enzymes › Hydrolases › Esterases › Carboxylic ester hydrolases › Phospholipases A › Phospholipases A2 › cDNA cloning, heterologous expression, protein...
questionsmedicales.fr › Phénomènes chimiques › Phénomènes biochimiques › Pliage des protéines › cDNA cloning, heterologous expression, protein...