3-D physiomimetic extracellular matrix hydrogels provide a supportive microenvironment for rodent and human islet culture.
3-D Hydrogels
Decellularized tissues
Islet niche
Journal
Biomaterials
ISSN: 1878-5905
Titre abrégé: Biomaterials
Pays: Netherlands
ID NLM: 8100316
Informations de publication
Date de publication:
04 2019
04 2019
Historique:
received:
05
04
2018
revised:
31
07
2018
accepted:
27
08
2018
pubmed:
19
9
2018
medline:
1
7
2020
entrez:
19
9
2018
Statut:
ppublish
Résumé
Organ-on-a-chip platforms serve as cost-efficient testbeds for screening pharmaceutical agents, mimicking natural physiology, and studying disease. In the field of diabetes, the development of an islet-on-a-chip platform would have broad implications in understanding disease pathology and discovering potential therapies. Islet microphysiological systems are limited, however, by their poor cell survival and function in culture. A key factor that has been implicated in this decline is the disruption of islet-matrix interactions following isolation. Herein, we sought to recapitulate the in vivo peri-islet niche using decellularized extracellular matrix (ECM) hydrogels. Sourcing from porcine bladder, lung, and pancreas tissues, 3-D ECM hydrogels were generated, characterized, and validated using both rodent and human pancreatic islets. Optimized decellularization protocols resulted in hydrogels with distinctive viscoelastic properties that correlated to their matrix composition. The in situ 3-D encapsulation of human or rat islets within ECM hydrogels resulted in improved functional stability over standard culture conditions. Islet composition and morphology were also altered, with enhanced retention of islet-resident endothelial cells and the formation of cord-like structures or sprouts emerging from the islet spheroid. These supportive 3-D physiomimetic ECM hydrogels can be leveraged within microfluidic platforms for the long-term culture of islets.
Identifiants
pubmed: 30224090
pii: S0142-9612(18)30617-3
doi: 10.1016/j.biomaterials.2018.08.057
pmc: PMC6397100
mid: NIHMS1506842
pii:
doi:
Substances chimiques
Hydrogels
0
Types de publication
Journal Article
Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
37-48Subventions
Organisme : NIDDK NIH HHS
ID : U24 DK098085
Pays : United States
Organisme : NIDDK NIH HHS
ID : UC4 DK098085
Pays : United States
Organisme : NIDDK NIH HHS
ID : UC4 DK104208
Pays : United States
Informations de copyright
Copyright © 2018 Elsevier Ltd. All rights reserved.
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