Development of a method for quantitative determination of the cytotoxic agent piplartine (piperlongumine) in multiple skin layers.
HPLC quantification
cutaneous bioavailability
piperlongumine
piplartine
skin
Journal
Biomedical chromatography : BMC
ISSN: 1099-0801
Titre abrégé: Biomed Chromatogr
Pays: England
ID NLM: 8610241
Informations de publication
Date de publication:
Feb 2019
Feb 2019
Historique:
received:
07
08
2018
revised:
07
09
2018
accepted:
13
09
2018
pubmed:
22
9
2018
medline:
26
2
2019
entrez:
22
9
2018
Statut:
ppublish
Résumé
This study reports the development of a simple and reproducible method, with high rates of recovery, to extract the cytotoxic agent piplartine from skin layers, and a sensitive and rapid UV-HPLC method for its quantification. Considering the potential of piplartine for topical treatment of skin cancer, this method may find application for formulation development and pharmacokinetics studies to assess cutaneous bioavailability. Porcine skin was employed as a model for human tissue. Piplartine was extracted from the stratum corneum (SC) and remaining viable skin layers (VS) using methanol, vortex homogenization and bath sonication, and subsequently assayed by HPLC using a C
Substances chimiques
Antineoplastic Agents, Phytogenic
0
Dioxolanes
0
Piperidones
0
piperlongumine
SGD66V4SVJ
piperlongumine
SGD66V4SVJ
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
e4386Subventions
Organisme : Brazilian National Council for Technological and Scientific Development
ID : 443549/2014-1
Organisme : São Paulo Research Foundation
ID : 2013/16617-7
Organisme : Fellowships from Brazilian National Council for Technological and Scientific Development, Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (Finance code 001) and São Paulo Research Foundation
ID : 2017/04174-4
Organisme : Fellowships from Brazilian National Council for Technological and Scientific Development, Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (Finance code 001) and São Paulo Research Foundation
ID : 2017/07856-9
Informations de copyright
© 2018 John Wiley & Sons, Ltd.