Distinct microRNA and mRNA responses elicited by ecdysone, diapause hormone and a diapause hormone analog at diapause termination in pupae of the corn earworm, Helicoverpa zea.
Journal
General and comparative endocrinology
ISSN: 1095-6840
Titre abrégé: Gen Comp Endocrinol
Pays: United States
ID NLM: 0370735
Informations de publication
Date de publication:
01 07 2019
01 07 2019
Historique:
received:
21
05
2018
revised:
10
09
2018
accepted:
19
09
2018
pubmed:
24
9
2018
medline:
17
10
2019
entrez:
24
9
2018
Statut:
ppublish
Résumé
Ecdysone, diapause hormone and a diapause hormone analog are all capable of breaking pupal diapause and prompting initiation of adult development in the cotton earworm, Helicoverpa zea. In this study we asked whether these three chemically-distinct diapause terminators elicit the same effect on expression of a collection of microRNAs and transcripts encoding components of the ecdysone signaling pathway. Injection of all three endocrine agents resulted in downregulation of one miRNA, miR-277-3p, a miRNA previously linked to the insulin/FOXO signaling pathway, and all three agents promoted upregulation of spook, a member of the ecdysone biosynthesis pathway, and iswi, an ecdysone-responsive transcript. Other miRNA and mRNA responses varied depending on the agent used to terminate diapause, thus suggesting that different endocrine pathways and mechanisms can lead to the same final developmental response.
Identifiants
pubmed: 30243885
pii: S0016-6480(18)30316-2
doi: 10.1016/j.ygcen.2018.09.013
pii:
doi:
Substances chimiques
MicroRNAs
0
Neuropeptides
0
RNA, Messenger
0
Ecdysone
3604-87-3
Ecdysterone
5289-74-7
diapause hormone
9086-74-2
Types de publication
Journal Article
Research Support, U.S. Gov't, Non-P.H.S.
Langues
eng
Sous-ensembles de citation
IM
Pagination
68-78Informations de copyright
Copyright © 2018 Elsevier Inc. All rights reserved.