A newly developed tetraplex real-time RT-PCR for simultaneous screening of influenza virus types A, B, C and D.
Animals
DNA Primers
/ genetics
Epidemiological Monitoring
/ veterinary
Europe
High-Throughput Screening Assays
/ methods
Influenza A virus
/ isolation & purification
Influenza B virus
/ isolation & purification
Gammainfluenzavirus
/ isolation & purification
Multiplex Polymerase Chain Reaction
/ methods
Orthomyxoviridae
/ isolation & purification
Orthomyxoviridae Infections
/ diagnosis
RNA, Viral
/ genetics
Sensitivity and Specificity
Sus scrofa
Swine
Swine Diseases
/ virology
Thogotovirus
/ isolation & purification
European surveillance
influenza virus types A, B, C and D
multiplex RT-qPCR
swine
Journal
Influenza and other respiratory viruses
ISSN: 1750-2659
Titre abrégé: Influenza Other Respir Viruses
Pays: England
ID NLM: 101304007
Informations de publication
Date de publication:
01 2019
01 2019
Historique:
received:
07
06
2018
revised:
12
09
2018
accepted:
13
09
2018
pubmed:
29
9
2018
medline:
9
11
2019
entrez:
29
9
2018
Statut:
ppublish
Résumé
Human- or avian-to-swine transmissions have founded several autonomously circulating influenza A virus (IAV) lineages in swine populations that cause economically important respiratory disease. Little is known on other human influenza virus types, like B (IBV) and C (ICV) in European swine, and of the recently detected novel animal influenza virus type D (IDV). Development of a cost-effective diagnostic tool for large-scale surveillance programmes targeting all four influenza virus types. An influenza ABCD tetraplex real-time RT-PCR (RT-qPCR) was developed in the frame of this study. A selection of reference virus strains and more than 4000 porcine samples from a passive IAV surveillance programme in European swine with acute respiratory disease were examined. Two IBV, a single IDV but no ICV infections were identified by tetraplex RT-qPCR. IBV and IDV results were confirmed by conventional RT-PCR and partial sequence analysis. The tetraplex RT-qPCR proved fit for purpose as a sensitive, specific and high-throughput tool to study influenza virus transmission at the human-animal interface. Complementing close-meshed active virological and serological surveillance is required to better understand the true incidence and prevalence of influenza virus type B, C and D infections in swine.
Sections du résumé
BACKGROUND
Human- or avian-to-swine transmissions have founded several autonomously circulating influenza A virus (IAV) lineages in swine populations that cause economically important respiratory disease. Little is known on other human influenza virus types, like B (IBV) and C (ICV) in European swine, and of the recently detected novel animal influenza virus type D (IDV).
OBJECTIVES
Development of a cost-effective diagnostic tool for large-scale surveillance programmes targeting all four influenza virus types.
METHODS
An influenza ABCD tetraplex real-time RT-PCR (RT-qPCR) was developed in the frame of this study. A selection of reference virus strains and more than 4000 porcine samples from a passive IAV surveillance programme in European swine with acute respiratory disease were examined.
RESULTS
Two IBV, a single IDV but no ICV infections were identified by tetraplex RT-qPCR. IBV and IDV results were confirmed by conventional RT-PCR and partial sequence analysis.
CONCLUSIONS
The tetraplex RT-qPCR proved fit for purpose as a sensitive, specific and high-throughput tool to study influenza virus transmission at the human-animal interface. Complementing close-meshed active virological and serological surveillance is required to better understand the true incidence and prevalence of influenza virus type B, C and D infections in swine.
Identifiants
pubmed: 30264926
doi: 10.1111/irv.12613
pmc: PMC6304318
doi:
Substances chimiques
DNA Primers
0
RNA, Viral
0
Banques de données
GENBANK
['EPI240880', 'CY045781', 'EPI248511', 'EPI248525', 'FJ970928', 'EPI356425', 'EPI236901', 'DQ102484', 'EPI411926', 'EPI411955', 'EPI411878', 'EPI99278', 'DQ186977', 'EPI411916', 'EPI411892', 'EPI356461', 'EPI301660', 'EPI174502', 'EU478801', 'EPI411978', 'EPI411965', 'EPI4555', 'AF059988', 'EPI159946', 'CY033844', 'KC892118', 'EPI175755', 'CY038287', 'EPI173277', 'FJ766840', 'EPI283751', 'FR671424', 'EPI230654', 'AY880247', 'EPI816669', 'LC123816', 'EPI231872', 'AB099600', 'EPI231604', 'AB099602', 'EPI231953', 'AB099582', 'EPI816678', 'LC123825', 'EPI816685', 'LC123832', 'EPI816695', 'LC123842', 'EPI816706', 'LC123853', 'EPI816712', 'LC123859', 'KT592534', 'JQ922309']
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
71-82Informations de copyright
© 2018 The Authors. Influenza and Other Respiratory Viruses Published by John Wiley & Sons Ltd.
Références
mBio. 2014 Mar 04;5(2):e00031-14
pubmed: 24595369
Curr Top Microbiol Immunol. 2013;370:201-25
pubmed: 23023606
J Clin Virol. 2016 Aug;81:31-3
pubmed: 27294672
Influenza Other Respir Viruses. 2010 Sep;4(5):277-93
pubmed: 20716157
J Virol. 2008 Jul;82(13):6419-26
pubmed: 18448539
Curr Top Microbiol Immunol. 2014;385:307-26
pubmed: 25033752
J Virol. 2015 Oct;89(19):9920-31
pubmed: 26202246
Avian Dis. 2003;47(3 Suppl):1079-82
pubmed: 14575115
Arch Virol. 2014 Feb;159(2):249-55
pubmed: 23942954
PLoS Pathog. 2013 Feb;9(2):e1003176
pubmed: 23408893
J Infect Dis. 2018 Jan 30;217(4):548-559
pubmed: 29325138
J Virol. 2015 May;89(9):4818-26
pubmed: 25673727
Sci Rep. 2017 Sep 15;7(1):11660
pubmed: 28916759
Curr Top Microbiol Immunol. 2013;370:29-55
pubmed: 23011571
Zoonoses Public Health. 2014 Feb;61(1):4-17
pubmed: 23556412
Influenza Other Respir Viruses. 2019 Jan;13(1):91-105
pubmed: 29996007
Nature. 2009 Jun 18;459(7249):931-9
pubmed: 19525932
J Clin Virol. 2016 Jan;74:50-3
pubmed: 26655269
Nucleic Acids Res. 2007 Jul;35(Web Server issue):W43-6
pubmed: 17452344
Trends Microbiol. 2015 Mar;23(3):142-53
pubmed: 25564096
Science. 2009 Jul 10;325(5937):197-201
pubmed: 19465683
J Gen Virol. 2012 Oct;93(Pt 10):2195-2203
pubmed: 22791604
Arch Gesamte Virusforsch. 1951;4(4):485-500
pubmed: 13249484
Virology. 1990 Mar;175(1):59-68
pubmed: 2309452
Science. 1950 Oct 27;112(2913):495-7
pubmed: 14787444
PLoS Pathog. 2013;9(10):e1003657
pubmed: 24130481
PLoS One. 2014 Dec 26;9(12):e115815
pubmed: 25542013
Eur J Clin Microbiol Infect Dis. 2016 Jun;35(6):941-6
pubmed: 26980094
Influenza Other Respir Viruses. 2019 Jan;13(1):71-82
pubmed: 30264926
J Gen Virol. 1983 Jan;64 (Pt 1):177-82
pubmed: 6296296
Philos Trans R Soc Lond B Biol Sci. 2001 Dec 29;356(1416):1861-70
pubmed: 11779385
Curr Top Microbiol Immunol. 2013;370:1-20
pubmed: 23124938
Emerg Infect Dis. 2015 Feb;21(2):368-71
pubmed: 25628038
Nature. 1969 Apr 12;222(5189):184-5
pubmed: 4304929
Methods Mol Biol. 2014;1161:243-58
pubmed: 24899434
Berl Munch Tierarztl Wochenschr. 2010 Jul-Aug;123(7-8):286-92
pubmed: 20690540
PLoS One. 2010 Feb 05;5(2):e9068
pubmed: 20140096
Microb Genom. 2016 Nov 30;2(11):e000093
pubmed: 28348833
PLoS Pathog. 2017 Dec 28;13(12):e1006749
pubmed: 29284042
Influenza Other Respir Viruses. 2011 Mar;5(2):110-4
pubmed: 21306574
Emerg Infect Dis. 2018 Jul;24(7):1388-1389
pubmed: 29912692
J Virol. 2015 Jan 15;89(2):1036-42
pubmed: 25355894