Protein Kinase R Is Constitutively Expressed in the Human Pancreas.
PCR
immunohistochemistry
innate immunity
pancreas
type 1 diabetes
viral footprints
viral sensors
Journal
The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society
ISSN: 1551-5044
Titre abrégé: J Histochem Cytochem
Pays: United States
ID NLM: 9815334
Informations de publication
Date de publication:
02 2019
02 2019
Historique:
pubmed:
29
9
2018
medline:
5
11
2019
entrez:
29
9
2018
Statut:
ppublish
Résumé
Viral infection of the insulin-producing cells in the pancreas has been proposed in the etiology of type 1 diabetes. Protein kinase R (PKR) is a cytoplasmic protein activated through phosphorylation in response to cellular stress and particularly viral infection. As PKR expression in pancreatic beta-cells has been interpreted as a viral footprint, this cross-sectional study aimed at characterizing the PKR expression in non-diabetic human pancreases. PKR expression was evaluated in pancreas tissue from 16 non-diabetic organ donors, using immunohistochemistry, qPCR, and western blot. Immunohistochemistry and western blot showed readily detectable PKR expression in the pancreatic parenchyma. The qPCR detected PKR mRNA in both endocrine and exocrine samples, with a slightly higher expression in the islets. In conclusion, PKR is constitutively expressed in both endocrine and exocrine parts of the pancreas and its expression should not be interpreted as a viral footprint in pancreatic beta cells.
Identifiants
pubmed: 30265185
doi: 10.1369/0022155418802838
pmc: PMC6354319
doi:
Substances chimiques
RNA, Messenger
0
eIF-2 Kinase
EC 2.7.11.1
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
99-105Références
EMBO J. 1997 Mar 17;16(6):1291-304
pubmed: 9135145
J Virol. 1993 Dec;67(12):7695-700
pubmed: 7693978
PLoS Pathog. 2011 Sep;7(9):e1002267
pubmed: 21977009
Diabetologia. 2009 Jun;52(6):1143-51
pubmed: 19266182
Open Virol J. 2013 Apr 05;7:49-56
pubmed: 23723955
J Biol Chem. 2002 Sep 6;277(36):33058-67
pubmed: 12060652
J Virol. 1998 Dec;72(12):9934-9
pubmed: 9811730
Diabetes. 2016 Oct;65(10):3104-10
pubmed: 27422384
Cell. 1990 Jul 27;62(2):379-90
pubmed: 1695551
Mol Oncol. 2014 Jun;8(4):783-98
pubmed: 24725481
Diabetes. 2015 May;64(5):1682-7
pubmed: 25422108
J Biol Chem. 1993 Feb 15;268(5):3017-20
pubmed: 8428975
Mol Cell Biol. 1999 Jul;19(7):4653-63
pubmed: 10373514
EMBO J. 2001 May 15;20(10):2487-96
pubmed: 11350938
Proc Natl Acad Sci U S A. 1979 Feb;76(2):600-4
pubmed: 284384
Proc Natl Acad Sci U S A. 1993 May 15;90(10):4621-5
pubmed: 8099444
Transplantation. 2004 Nov 15;78(9):1367-75
pubmed: 15548977
Diabetes. 2008 Nov;57(11):2863-71
pubmed: 18971433
Diabetologia. 2013 Jan;56(1):185-93
pubmed: 23064357