An Innovative Multiplexed and Flexible Molecular Approach for the Differential Detection of Arboviruses.
Chikungunya Fever
/ diagnosis
Chikungunya virus
/ genetics
Dengue
/ diagnosis
Dengue Virus
/ genetics
Humans
Multiplex Polymerase Chain Reaction
/ methods
Nucleic Acid Hybridization
/ methods
Reverse Transcriptase Polymerase Chain Reaction
/ methods
Sensitivity and Specificity
Zika Virus
/ genetics
Zika Virus Infection
/ diagnosis
Journal
The Journal of molecular diagnostics : JMD
ISSN: 1943-7811
Titre abrégé: J Mol Diagn
Pays: United States
ID NLM: 100893612
Informations de publication
Date de publication:
01 2019
01 2019
Historique:
received:
13
04
2018
revised:
20
07
2018
accepted:
13
08
2018
pubmed:
1
10
2018
medline:
19
6
2020
entrez:
1
10
2018
Statut:
ppublish
Résumé
Nucleic acid testing during the preseroconversion viremic phase is required to differentially diagnose arboviral infections. The continuing emergence of arboviruses, such as Zika virus (ZIKV), dengue virus (DENV), and chikungunya virus (CHIKV), necessitates the development of a flexible diagnostic approach. Similar clinical signs and the priority to protect pregnant women from ZIKV infection indicate that the differential diagnosis of arboviruses is essential for effective patient management, clinical care, and epidemiologic surveillance. We describe an innovative diagnostic approach that combines generic RT-PCR amplification and identification by hybridization to specific probes. Original tetrathiolated probes were designed for the robust, sensitive, and specific detection of amplified arboviral genomes. The limit of detection using cultured and quantified stocks of whole viruses was 1 TCID
Identifiants
pubmed: 30268947
pii: S1525-1578(18)30157-0
doi: 10.1016/j.jmoldx.2018.08.005
pii:
doi:
Types de publication
Evaluation Study
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
81-88Informations de copyright
Copyright © 2019 American Society for Investigative Pathology and the Association for Molecular Pathology. Published by Elsevier Inc. All rights reserved.