Using of keratin substrate for enrichment of HT29 colorectal cancer stem-like cells.
cancer stem cell
colorectal cancer
keratin substrate
spheroid
Journal
Journal of biomedical materials research. Part B, Applied biomaterials
ISSN: 1552-4981
Titre abrégé: J Biomed Mater Res B Appl Biomater
Pays: United States
ID NLM: 101234238
Informations de publication
Date de publication:
05 2019
05 2019
Historique:
received:
20
03
2018
revised:
08
07
2018
accepted:
02
08
2018
pubmed:
4
10
2018
medline:
15
7
2020
entrez:
4
10
2018
Statut:
ppublish
Résumé
Eradication of cancer stem-like cells (CSLCs) are becoming increasingly an important target for new cancer therapies. The ability to study their behavior in vitro will provide the opportunity for high-throughput testing of more effective treatments. In this study, spheroid-like structures' formation and enrichment of HT29 CSLCs were evaluated on a wool keratin-based substrate as a bio-mimic of natural extracellular matrix (ECM) proteins. The results indicated that culturing on keratin substrate increased spheroid formation ability and radio-/chemoresistance of HT29 cells. Moreover, cell surface expression of CD133 CSLCs' marker and the mRNA level of stemness genes such as Nanog, Oct4, and c-MYC were increased. These data suggest that keratin can potentially be used for spheroid-like structure formation and enrichment of HT29 CSLCs. In addition, it seems that the induction of stemness characteristics on keratin substrate is probably because of the activation of α
Substances chimiques
Biomarkers, Tumor
0
Keratins
68238-35-7
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
1264-1271Subventions
Organisme : Metabolic Disorders Research Center
Pays : International
Organisme : Golestan University of Medical Science
ID : 950616136
Pays : International
Informations de copyright
© 2018 Wiley Periodicals, Inc.