Miniscope GRIN Lens System for Calcium Imaging of Neuronal Activity from Deep Brain Structures in Behaving Animals.
GCaMP6
GRadient
INdex lens
calcium imaging
freely behaving mice
miniscope
Journal
Current protocols in neuroscience
ISSN: 1934-8576
Titre abrégé: Curr Protoc Neurosci
Pays: United States
ID NLM: 9706581
Informations de publication
Date de publication:
01 2019
01 2019
Historique:
pubmed:
14
10
2018
medline:
8
5
2019
entrez:
14
10
2018
Statut:
ppublish
Résumé
Visualizing neural activity from deep brain regions in freely behaving animals through miniature fluorescent microscope (miniscope) systems is becoming more important for understanding neural encoding mechanisms underlying cognitive functions. Here we present our custom-designed miniscope GRadient INdex (GRIN) lens system that enables simultaneously recording from hundreds of neurons for months. This article includes miniscope design, the surgical procedure for GRIN lens implantation, miniscope mounting on the head of a mouse, and data acquisition and analysis. First, a target brain region is labeled with virus expressing GCaMP6; second, a GRIN lens is implanted above the target brain region; third, following mouse surgical recovery, a miniscope is mounted on the head of the mouse above the GRIN lens; and finally, neural activity is recorded from the freely behaving mouse. This system can be applied to recording the same population of neurons longitudinally, enabling the elucidation of neural mechanisms underlying behavioral control. © 2018 by John Wiley & Sons, Inc.
Identifiants
pubmed: 30315730
doi: 10.1002/cpns.56
pmc: PMC6318019
mid: NIHMS988635
doi:
Substances chimiques
Calcium
SY7Q814VUP
Types de publication
Journal Article
Research Support, N.I.H., Intramural
Langues
eng
Sous-ensembles de citation
IM
Pagination
e56Subventions
Organisme : NIGMS NIH HHS
ID : P20 GM121310
Pays : United States
Organisme : Intramural NIH HHS
ID : ZIA DA000603-03
Pays : United States
Informations de copyright
© 2018 John Wiley & Sons, Inc.
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