Does lipidomic serum analysis support the assessment of digestive efficiency in chickens?


Journal

Poultry science
ISSN: 1525-3171
Titre abrégé: Poult Sci
Pays: England
ID NLM: 0401150

Informations de publication

Date de publication:
01 Mar 2019
Historique:
received: 21 02 2018
accepted: 19 09 2018
pubmed: 17 10 2018
medline: 12 3 2019
entrez: 17 10 2018
Statut: ppublish

Résumé

The increasing cost of conventional feedstuffs used in poultry diets has bolstered interest in genetic selection for digestive efficiency (DE) to improve the adaptation of the birds to various alternative feedstuffs. However, DE measurement through AMEn is time-consuming and constraining. To simplify selection for DE, the potential of serum composition to predict AMEn was evaluated based on 40 birds from two broiler lines (D+ and D-) divergently selected on the fecal AMEn of a difficult-to-digest wheat-based diet. Differences in serum coloration were suspected between the two lines, and thus a spectrophotometric analysis was carried out, revealing a significant difference in absorption between 430 nm and 516 nm, corresponding to the signature of orange-red lipophilic pigments such as xanthophylls. To go further, the liposoluble fraction of the serum was explored for its lipidome by mass spectrometry. Discriminant analysis revealed that a pattern of 10 metabolites, including zeaxanthin/lutein, can explain 82% of the lipidomic differences between the two lines. Colorimetry combined with lipidomics studies confirmed the relationship between digestive efficiency and serum composition, which opens up new possibilities for using it as a quick and easy proxy of digestive efficiency.

Identifiants

pubmed: 30325459
pii: S0032-5791(19)30416-X
doi: 10.3382/ps/pey483
pmc: PMC6377433
doi:

Substances chimiques

Lipids 0

Types de publication

Journal Article

Langues

eng

Sous-ensembles de citation

IM

Pagination

1425-1431

Informations de copyright

© The Author(s) 2018. Published by Oxford University Press on behalf of Poultry Science Association.

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Auteurs

Stéphane Beauclercq (S)

BOA, INRA, Université de Tours, 37380 Nouzilly, France.

Antoine Lefèvre (A)

Université de Tours, PST Analyse des systèmes biologiques, 37044 Tours Cedex 9, Tours, France.

Lydie Nadal-Desbarats (L)

Université de Tours, PST Analyse des systèmes biologiques, 37044 Tours Cedex 9, Tours, France.
UMR 1253, iBrain, Université de Tours, Inserm, 37032 Tours cedex 1, Tours, France.

Karine Germain (K)

INRA, UE 1206 Elevage Alternatif et Santé des Monogastriques, Le Magneraud, Saint-Pierre-d'Amilly, BP 52, 17700 Surgères, France.

Christophe Praud (C)

BOA, INRA, Université de Tours, 37380 Nouzilly, France.

Patrick Emond (P)

Université de Tours, PST Analyse des systèmes biologiques, 37044 Tours Cedex 9, Tours, France.
UMR 1253, iBrain, Université de Tours, Inserm, 37032 Tours cedex 1, Tours, France.
CHRU de Tours, Service de Médecine Nucléaire In Vitro, 37032 Tours cedex, Tours, France.

Elisabeth Le Bihan-Duval (EL)

BOA, INRA, Université de Tours, 37380 Nouzilly, France.

Sandrine Mignon-Grasteau (S)

BOA, INRA, Université de Tours, 37380 Nouzilly, France.

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