Subcellular antigen localization in commensal E. coli is critical for T cell activation and induction of specific tolerance.
Administration, Oral
Animals
Antigens, Bacterial
/ genetics
Cells, Cultured
Escherichia coli
/ physiology
Female
Forkhead Transcription Factors
/ metabolism
Gastrointestinal Microbiome
/ physiology
Homeostasis
Immune Tolerance
Intracellular Space
Lymphocyte Activation
Mice
Mice, Inbred C57BL
Mice, Transgenic
Microorganisms, Genetically-Modified
Ovalbumin
/ genetics
Peptides
/ genetics
Symbiosis
T-Lymphocytes, Regulatory
/ immunology
Journal
Mucosal immunology
ISSN: 1935-3456
Titre abrégé: Mucosal Immunol
Pays: United States
ID NLM: 101299742
Informations de publication
Date de publication:
01 2019
01 2019
Historique:
received:
11
06
2017
accepted:
23
06
2018
revised:
17
06
2018
pubmed:
18
10
2018
medline:
15
5
2019
entrez:
18
10
2018
Statut:
ppublish
Résumé
Oral tolerance to soluble antigens is critically important for the maintenance of immunological homeostasis in the gut. The mechanisms of tolerance induction to antigens of the gut microbiota are still less well understood. Here, we investigate whether the subcellular localization of antigens within non-pathogenic E. coli has a role for its ability to induce antigen-specific tolerance. E. coli that express an ovalbumin (OVA) peptide in the cytoplasm, at the outer membrane or as secreted protein were generated. Intestinal colonization of mice with non-pathogenic E. coli expressing OVA at the membrane induced the expansion of antigen-specific Foxp3
Identifiants
pubmed: 30327531
doi: 10.1038/s41385-018-0061-0
pii: S1933-0219(22)00358-0
doi:
Substances chimiques
Antigens, Bacterial
0
FOXP3 protein, human
0
Forkhead Transcription Factors
0
Peptides
0
Ovalbumin
9006-59-1
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
97-107Références
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