Biomedical potential of chitosan-silver nanoparticles with special reference to antioxidant, antibacterial, hemolytic and in vivo cutaneous wound healing effects.
Animals
Anti-Bacterial Agents
/ pharmacology
Antioxidants
/ pharmacology
Biphenyl Compounds
/ chemistry
CHO Cells
Chitosan
/ chemistry
Cricetinae
Cricetulus
Escherichia coli
/ drug effects
Free Radical Scavengers
/ pharmacology
Free Radicals
Gels
Hemolysis
Humans
Iron
/ chemistry
Linoleic Acid
/ chemistry
Male
Metal Nanoparticles
/ chemistry
Microbial Sensitivity Tests
Oxidative Stress
Oxygen
/ chemistry
Picrates
/ chemistry
Rats
Rats, Wistar
Silver
/ pharmacology
Silver Nitrate
/ pharmacology
Skin
/ drug effects
Staphylococcus aureus
/ drug effects
Wound Healing
beta Carotene
/ chemistry
Antioxidant and antibacterial activities
Chitosan-silver nanoparticles
Cytotoxicity effect
Structural characterization
Wound-healing efficiency
Journal
Biochimica et biophysica acta. General subjects
ISSN: 1872-8006
Titre abrégé: Biochim Biophys Acta Gen Subj
Pays: Netherlands
ID NLM: 101731726
Informations de publication
Date de publication:
01 2019
01 2019
Historique:
received:
27
06
2018
revised:
03
10
2018
accepted:
13
10
2018
pubmed:
20
10
2018
medline:
17
8
2019
entrez:
20
10
2018
Statut:
ppublish
Résumé
The aim of the present study was to prepare chitosan-PVA-silver nanoparticles (CS-AgNPs) through green method. Chitosan and PVA polymers acted as stabilizing agents. DLS and TEM analyses showed that CS-AgNPs were homogeneously dispersed in matrix with an average size of 190-200 nm. The CS-AgNPs were tested for their antioxidant and antibacterial properties and the results revealed that they exhibited higher antioxidant activity than CS powder. Moreover, CS-AgNPs were characterized by a low cytotoxicity effect at 5-200 μg/ml against Chinese Hamster Ovary (CHO-K1) cells. In addition, the prepared CS-Ag NPs were found to promote significantly the wound healing, as determined by the wound contraction ratio and histological examination. A significant improvement in wound healing progression and in oxidative stress damage were observed for CS, CS-PVA and CS-AgNPs-treated wound tissues, when compared to control and CICAFLORA®-treated groups. The wound healing effect could be attributed to the antibacterial and antioxidant synergy of AgNPs and CS. Results strongly support the possibility of using CS-AgNPs for wound care applications.
Identifiants
pubmed: 30339915
pii: S0304-4165(18)30331-3
doi: 10.1016/j.bbagen.2018.10.010
pii:
doi:
Substances chimiques
Anti-Bacterial Agents
0
Antioxidants
0
Biphenyl Compounds
0
Free Radical Scavengers
0
Free Radicals
0
Gels
0
Picrates
0
beta Carotene
01YAE03M7J
Silver
3M4G523W1G
Chitosan
9012-76-4
Silver Nitrate
95IT3W8JZE
Linoleic Acid
9KJL21T0QJ
1,1-diphenyl-2-picrylhydrazyl
DFD3H4VGDH
Iron
E1UOL152H7
Oxygen
S88TT14065
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Pagination
241-254Informations de copyright
Copyright © 2018 Elsevier B.V. All rights reserved.