Primary structure and anticoagulant activity of fucoidan from the sea cucumber Holothuria polii.
Anticoagulant activity
Fucoidan
Holothuria polii
Sea cucumber
Thrombin generation
Journal
International journal of biological macromolecules
ISSN: 1879-0003
Titre abrégé: Int J Biol Macromol
Pays: Netherlands
ID NLM: 7909578
Informations de publication
Date de publication:
Jan 2019
Jan 2019
Historique:
received:
31
07
2018
revised:
18
09
2018
accepted:
15
10
2018
pubmed:
20
10
2018
medline:
18
1
2019
entrez:
20
10
2018
Statut:
ppublish
Résumé
Sea cucumber fucoidan is recently endowed with a variety of biological activities. In the present study, we studied the structure and anticoagulant effect of fucoidan from the sea cucumber Holothuria polii (Fuc-Hp). The Fuc-Hp was purified by anion exchange chromatography and its structure was characterized by FT-IR and NMR spectroscopy. Molecular weight measurements were performed by HPSEC-MALLS-dRI. Fuc-Hp anticoagulant activity was measured by activated partial thromboplastin and thrombin times, and by in vitro thrombin inhibition in the presence of antithrombin and heparin cofactor II. The assessment of thrombin generation was investigated using calibrated automated thrombography. Fuc-Hp with a high sulfate content (34.6%) and an average molecular mass of 1376.3 kDa was isolated from H. polii in amount of ~2.7 mg/g dry body wall. Primary structural analysis indicated that Fuc-Hp was mainly composed of a tetrafucose repeating unit branched by glucuronic acid. Fuc-Hp exhibited a high anticoagulant effect mediated essentially by heparin cofactor II and to lesser extent by antithrombin with IC
Identifiants
pubmed: 30340002
pii: S0141-8130(18)32965-9
doi: 10.1016/j.ijbiomac.2018.10.129
pii:
doi:
Substances chimiques
Anticoagulants
0
Polysaccharides
0
fucoidan
9072-19-9
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
1145-1153Informations de copyright
Copyright © 2018 Elsevier B.V. All rights reserved.