Serum amyloid A does not affect high-density lipoprotein cholesterol measurement by a homogeneous assay.

Cholesterol, HDL / blood Female Humans Male Serum Amyloid A Protein / chemistry
High-density lipoprotein Inflammation Laboratory test Particle size Serum amyloid A Ultracentrifugation

Journal

Clinical biochemistry
ISSN: 1873-2933
Titre abrégé: Clin Biochem
Pays: United States
ID NLM: 0133660

Informations de publication

Date de publication:
Jan 2019
Historique:
received: 20 05 2018
revised: 13 10 2018
accepted: 17 10 2018
pubmed: 21 10 2018
medline: 12 1 2019
entrez: 21 10 2018
Statut: ppublish

Résumé

Serum amyloid A (SAA), which is one of the acute phase proteins, alters the structure of HDL by associating with it during circulation. We focused on whether SAA influences the values of HDL-cholesterol (HDL-C) measurements when using a homogeneous assay. HDLs were isolated by ultracentrifugation from serum samples of 248 patients that were stratified into three groups based on their serum SAA concentrations (low: SAA ≤ 8 μg/mL; middle: 8 < SAA ≤ 100 μg/mL; and high: SAA > 100 μg/mL). HDL-C concentrations of the serum samples measured by the homogeneous assay were compared with the total cholesterol concentrations of HDL fractions isolated by ultracentrifugation. HDLs obtained from patients with low SAA concentrations were separated into their general particle sizes and classified as HDL A large amount of SAA attached to HDL altered the HDL particle size but did not essentially affect HDL-C measurement by homogeneous assay.

Sections du résumé

BACKGROUND BACKGROUND
Serum amyloid A (SAA), which is one of the acute phase proteins, alters the structure of HDL by associating with it during circulation. We focused on whether SAA influences the values of HDL-cholesterol (HDL-C) measurements when using a homogeneous assay.
METHODS METHODS
HDLs were isolated by ultracentrifugation from serum samples of 248 patients that were stratified into three groups based on their serum SAA concentrations (low: SAA ≤ 8 μg/mL; middle: 8 < SAA ≤ 100 μg/mL; and high: SAA > 100 μg/mL). HDL-C concentrations of the serum samples measured by the homogeneous assay were compared with the total cholesterol concentrations of HDL fractions isolated by ultracentrifugation.
RESULTS RESULTS
HDLs obtained from patients with low SAA concentrations were separated into their general particle sizes and classified as HDL
CONCLUSIONS CONCLUSIONS
A large amount of SAA attached to HDL altered the HDL particle size but did not essentially affect HDL-C measurement by homogeneous assay.

Identifiants

DOI: 10.1016/j.clinbiochem.2018.10.008 PMID: 30342019
pubmed: 30342019
pii: S0009-9120(18)30529-0
doi: 10.1016/j.clinbiochem.2018.10.008
pii:
doi:

Substances chimiques

Cholesterol, HDL 0
Serum Amyloid A Protein 0

Types de publication

Journal Article

Langues

eng

Sous-ensembles de citation

IM

Pagination

97-101

Informations de copyright

Copyright © 2018 The Authors. Published by Elsevier Inc. All rights reserved.

Auteurs

Megumi Sato (M)

Department of Analytical Laboratory Chemistry, Graduate School of Medical and Dental Sciences, Tokyo Medical and Dental University (TMDU), 1-5-45 Yushima, Bunkyo-ku, Tokyo 113-8510, Japan; Department of Clinical Laboratory, The University of Tokyo Hospital, 7-3-1 Hongo, Bunkyo-ku, Tokyo 113-8655, Japan.

Ryunosuke Ohkawa (R)

Department of Analytical Laboratory Chemistry, Graduate School of Medical and Dental Sciences, Tokyo Medical and Dental University (TMDU), 1-5-45 Yushima, Bunkyo-ku, Tokyo 113-8510, Japan. Electronic address: ryu-th@umin.ac.jp.

Hann Low (H)

Department of Lipoproteins and Atherosclerosis, Baker Heart and Diabetes Institute, 75 Commercial Rd, Melbourne, VIC 3004, Australia.

Madoka Nishimori (M)

Department of Clinical Laboratory, The University of Tokyo Hospital, 7-3-1 Hongo, Bunkyo-ku, Tokyo 113-8655, Japan.

Shigeo Okubo (S)

Faculty of Health Science Technology, Bunkyo Gakuin University, 1-19-1 Mukogaoka, Bunkyo-ku, Tokyo 113-8668, Japan.

Akira Yoshimoto (A)

Department of Analytical Laboratory Chemistry, Graduate School of Medical and Dental Sciences, Tokyo Medical and Dental University (TMDU), 1-5-45 Yushima, Bunkyo-ku, Tokyo 113-8510, Japan; Department of Clinical Laboratory, The University of Tokyo Hospital, 7-3-1 Hongo, Bunkyo-ku, Tokyo 113-8655, Japan.

Kouji Yano (K)

Center for Genomic and Regenerative Medicine, Graduate School of Medicine, Juntendo University, 2-1-1 Hongo, Bunkyo-ku, Tokyo 113-8421, Japan.

Takahiro Kameda (T)

Department of Medical Technology, School of Health Sciences, Tokyo University of Technology, 5-23-22 Nishikamata, Ota-ku, Tokyo 144-8535, Japan.

Yutaka Yatomi (Y)

Department of Clinical Laboratory, The University of Tokyo Hospital, 7-3-1 Hongo, Bunkyo-ku, Tokyo 113-8655, Japan.

Minoru Tozuka (M)

Department of Analytical Laboratory Chemistry, Graduate School of Medical and Dental Sciences, Tokyo Medical and Dental University (TMDU), 1-5-45 Yushima, Bunkyo-ku, Tokyo 113-8510, Japan.

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Classifications MeSH

questionsmedicales.fr › Acides aminés, peptides et protéines › Protéines › Lipoprotéines › Lipoprotéines HDL › Cholestérol HDL › Serum amyloid A does not affect high-density...
questionsmedicales.fr › Eucaryotes › Animaux › Chordés › Vertébrés › Mammifères › Eutheria › Primates › Haplorhini › Catarrhini › Hominidae › Humains › Serum amyloid A does not affect high-density...
questionsmedicales.fr › Acides aminés, peptides et protéines › Protéines › Protéines du sang › Protéine de la phase aigüe › Protéine amyloïde A sérique › Serum amyloid A does not affect high-density...