Effect of polysaccharide from Undaria pinnatifida on proliferation, migration and apoptosis of breast cancer cell MCF7.
Antineoplastic Agents
/ chemistry
Apoptosis
/ drug effects
Breast Neoplasms
/ pathology
Cell Movement
/ drug effects
Cell Proliferation
/ drug effects
Cell Survival
/ drug effects
Gene Expression Regulation, Neoplastic
/ drug effects
Humans
MCF-7 Cells
Polysaccharides
/ chemistry
Proliferating Cell Nuclear Antigen
/ metabolism
Undaria
/ chemistry
Anti-breast cancer
Polysaccharide
Undaria pinnatifida
Journal
International journal of biological macromolecules
ISSN: 1879-0003
Titre abrégé: Int J Biol Macromol
Pays: Netherlands
ID NLM: 7909578
Informations de publication
Date de publication:
Jan 2019
Jan 2019
Historique:
received:
01
09
2018
revised:
28
09
2018
accepted:
14
10
2018
pubmed:
22
10
2018
medline:
5
2
2019
entrez:
22
10
2018
Statut:
ppublish
Résumé
Sulfated polysaccharide from Undaria pinnatifida (SPUP) has significant anti-breast cancer activity. However, its anticarcinogenic mechanism still remains unclear. The aim of this article is to observe the effect of SPUP on proliferation, migration and apoptosis of human breast cancer cell line MCF7. Firstly, the effect of SPUP on proliferation was evaluated through MTT assay, plate clonality assay and immunofluorescence test of PCNA. The results showed that SPUP could significantly reduce MCF7 cells proliferation in a time- and dose-dependent manner. Based on transwell and scratch wound healing assays, then, inhibitory action of SPUP for MCF7 cells migration was observed. Finally, apoptosis and cycle arrest of SPUP for MCF7 cells also were found by the results from both flow cytometry analysis and Hoechst 33342 staining of apoptotic cells. Overall, these results showed anti-breast cancer mechanism of SPUP could be possibly related to inhibit migration, proliferation and induce apoptosis of cancer cells.
Identifiants
pubmed: 30342943
pii: S0141-8130(18)34643-9
doi: 10.1016/j.ijbiomac.2018.10.086
pii:
doi:
Substances chimiques
Antineoplastic Agents
0
Polysaccharides
0
Proliferating Cell Nuclear Antigen
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
734-742Informations de copyright
Copyright © 2018. Published by Elsevier B.V.