Studying Cell Mechanobiology in 3D: The Two-Photon Lithography Approach.
3D structures
cancer cell mechanobiology
stem cells
stiffness gradient
two-photon lithography
Journal
Trends in biotechnology
ISSN: 1879-3096
Titre abrégé: Trends Biotechnol
Pays: England
ID NLM: 8310903
Informations de publication
Date de publication:
04 2019
04 2019
Historique:
received:
11
05
2018
revised:
19
09
2018
accepted:
19
09
2018
pubmed:
23
10
2018
medline:
18
12
2019
entrez:
23
10
2018
Statut:
ppublish
Résumé
Two-photon lithography is a laser writing technique that can produce 3D microstructures with resolutions below the diffraction limit. This review focuses on its applications to study mechanical properties of cells, an emerging field known as mechanobiology. We review 3D structural designs and materials in the context of new experimental designs, including estimating forces exerted by single cells, studying selective adhesion on substrates, and creating 3D networks of cells. We then focus on emerging applications, including structures for assessing cancer cell invasiveness, whose migration properties depend on the cell mechanical response to the environment, and 3D architectures and materials to study stem cell differentiation, as 3D structure shape and patterning play a key role in defining cell fates.
Identifiants
pubmed: 30343948
pii: S0167-7799(18)30261-0
doi: 10.1016/j.tibtech.2018.09.008
pii:
doi:
Types de publication
Journal Article
Review
Langues
eng
Sous-ensembles de citation
IM
Pagination
358-372Informations de copyright
Copyright © 2018 Elsevier Ltd. All rights reserved.